Electrode surfaces based on multiwall carbon nanotubes-chitosan composites validated in the detection of homocysteine biomarkers for cardiovascular disease risk monitoring

Suparerk Oonchit, Benya Cherdhirunkorn, Phuntila Tharabenjasin, Noel Pabalan, Kumpol Chintanavilas, Robert Marks, Yardnapar Parcharoen, Chiravoot Pechyen

Research output: Contribution to journalArticlepeer-review

Abstract

This study aimed to modify screen-printed carbon micro-electrode surfaces by coating them with multiwall carbon-based nanotubes conjugated with chitosan and then validated the formed multiwall carbon-based nanotubes-chitosan coated screen printed carbon micro-electrode for the detection of homocysteine, a biomarker analyte known as a risk indicator in cardiovascular disease. The microstructure surface and crystallographic structure stability of the formed multiwall carbon-based nanotubes-chitosan obtained at formed multiwall carbon-based nanotubes per chitosan ratios of 1:1, 2:1, 3:1, and 4:1 were examined via field emission scanning electron microscopy, X-ray radiation, Raman spectroscopy, surface area and pore size, and thermogravimetric analyses. Homocysteine solutions at 30-100 μM were measured by cyclic voltammetry using the different formed multiwall carbon-based nanotubes-chitosan compositions as sensor electrodes. That with an optimal formed multiwall carbon-based nanotubes per chitosan ratio of 4:1 showed the highest crystallinity and electrical conductivity and gave a high coefficient of determination (R2 = 0.9036) between the homocysteine concentration and the oxidation current detection over an operating range of 30-100 μM. This new composite microelectrode for detecting homocysteine concentration makes it a promising candidate for clinical applications.

Original languageEnglish
Pages (from-to)144-154
Number of pages11
JournalEurobiotech Journal
Volume7
Issue number3
DOIs
StatePublished - 1 Jul 2023

Keywords

  • cardiovascular disease
  • homocysteine
  • sensor

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Molecular Medicine
  • Biomedical Engineering
  • Molecular Biology
  • Genetics

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