Electroenzymatic polypyrrole-intercalator sensor for the determination of West Nile virus cDNA

Serge Cosnier, Rodica E. Ionescu, Sebastien Herrmann, Laurent Bouffier, Martine Demeunynck, Robert S. Marks

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

The chemical binding of a redox acridone derivative onto a polypyrrole film functionalized by N-hydroxysuccinimide groups provided an electrode capable of anchoring DNA duplex by simple insertion of the grafted acridone intercalator into the dsDNA solution. This electrode was applied for the detection of a ssDNA derived from a West Nile virus sequence. The latter was thus amperometrically detected after its hybridization in solution with a biotinylated complementary oligonucleotide followed by its anchoring and labeling by a glucose oxidase at 1 pg/mL.

Original languageEnglish
Pages (from-to)7054-7057
Number of pages4
JournalAnalytical Chemistry
Volume78
Issue number19
DOIs
StatePublished - 1 Oct 2006

ASJC Scopus subject areas

  • Analytical Chemistry

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