Electroenzymatic polypyrrole-intercalator sensor for the determination of West Nile virus cDNA

Serge Cosnier; Rodica E. Ionescu; Sebastien Herrmann; Laurent Bouffier; Martine Demeunynck; Robert S. Marks

doi:10.1021/ac060926a

Electroenzymatic polypyrrole-intercalator sensor for the determination of West Nile virus cDNA

Serge Cosnier, Rodica E. Ionescu, Sebastien Herrmann, Laurent Bouffier, Martine Demeunynck, Robert S. Marks

Department of Biotechnology Engineering

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

The chemical binding of a redox acridone derivative onto a polypyrrole film functionalized by N-hydroxysuccinimide groups provided an electrode capable of anchoring DNA duplex by simple insertion of the grafted acridone intercalator into the dsDNA solution. This electrode was applied for the detection of a ssDNA derived from a West Nile virus sequence. The latter was thus amperometrically detected after its hybridization in solution with a biotinylated complementary oligonucleotide followed by its anchoring and labeling by a glucose oxidase at 1 pg/mL.

Original language	English
Pages (from-to)	7054-7057
Number of pages	4
Journal	Analytical Chemistry
Volume	78
Issue number	19
DOIs	https://doi.org/10.1021/ac060926a
State	Published - 1 Oct 2006

ASJC Scopus subject areas

Analytical Chemistry

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10.1021/ac060926a

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title = "Electroenzymatic polypyrrole-intercalator sensor for the determination of West Nile virus cDNA",

abstract = "The chemical binding of a redox acridone derivative onto a polypyrrole film functionalized by N-hydroxysuccinimide groups provided an electrode capable of anchoring DNA duplex by simple insertion of the grafted acridone intercalator into the dsDNA solution. This electrode was applied for the detection of a ssDNA derived from a West Nile virus sequence. The latter was thus amperometrically detected after its hybridization in solution with a biotinylated complementary oligonucleotide followed by its anchoring and labeling by a glucose oxidase at 1 pg/mL.",

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AU - Cosnier, Serge

AU - Ionescu, Rodica E.

AU - Herrmann, Sebastien

AU - Bouffier, Laurent

AU - Demeunynck, Martine

AU - Marks, Robert S.

PY - 2006/10/1

Y1 - 2006/10/1

N2 - The chemical binding of a redox acridone derivative onto a polypyrrole film functionalized by N-hydroxysuccinimide groups provided an electrode capable of anchoring DNA duplex by simple insertion of the grafted acridone intercalator into the dsDNA solution. This electrode was applied for the detection of a ssDNA derived from a West Nile virus sequence. The latter was thus amperometrically detected after its hybridization in solution with a biotinylated complementary oligonucleotide followed by its anchoring and labeling by a glucose oxidase at 1 pg/mL.

AB - The chemical binding of a redox acridone derivative onto a polypyrrole film functionalized by N-hydroxysuccinimide groups provided an electrode capable of anchoring DNA duplex by simple insertion of the grafted acridone intercalator into the dsDNA solution. This electrode was applied for the detection of a ssDNA derived from a West Nile virus sequence. The latter was thus amperometrically detected after its hybridization in solution with a biotinylated complementary oligonucleotide followed by its anchoring and labeling by a glucose oxidase at 1 pg/mL.

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JF - Industrial And Engineering Chemistry Analytical Edition

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Electroenzymatic polypyrrole-intercalator sensor for the determination of West Nile virus cDNA

Abstract

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