Electrophoretically deposited reduced graphene oxide platform for food toxin detection

Saurabh Srivastava, Vinod Kumar, Md Azahar Ali, Pratima R. Solanki, Anchal Srivastava, Gajjala Sumana, Preeti Suman Saxena, Amish G. Joshi, B. D. Malhotra

Research output: Contribution to journalArticlepeer-review

159 Scopus citations

Abstract

Reduced graphene oxide (RGO) due to its excellent electrochemical properties and large surface area, has recently aroused much interest for electrochemical biosensing application. Here, the chemically active RGO has been synthesized and deposited onto an indium tin oxide (ITO) coated glass substrate by the electrophoretic deposition technique. This novel platform has been utilized for covalent attachment of the monoclonal antibodies of aflatoxin B1 (anti-AFB1) for food toxin (AFB1) detection. The electron microscopy, X-ray diffraction, and UV-visible studies reveal successful synthesis of reduced graphene oxide while the XPS and FTIR studies suggest its carboxylic functionalized nature. The electrochemical sensing results of the anti-AFB1/RGO/ITO based immunoelectrode obtained as a function of aflatoxin concentration show high sensitivity (68 μA ng -1 mL cm-2) and improved detection limit (0.12 ng mL -1). The association constant (ka) for antigen-antibody interaction obtained as 5 × 10-4 ng mL-1 indicates high affinity of antibodies toward the antigen (AFB1).

Original languageEnglish
Pages (from-to)3043-3051
Number of pages9
JournalNanoscale
Volume5
Issue number7
DOIs
StatePublished - 1 Dec 2013
Externally publishedYes

ASJC Scopus subject areas

  • General Materials Science

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