Elevated concentrations of interleukin-6 in intra-amniotic infection with Ureaplasma urealyticum in asymptomatic women during genetic amniocentesis

Asher Bashiri, Shulamith Horowitz, Mahmud Huleihel, Rinat Hackmon, Doron Dukler, Moshe Mazor

Research output: Contribution to journalArticlepeer-review

43 Scopus citations


Background. Intra-amniotic infection is an important cause of preterm delivery and interleukin-6 (IL-6) determination was recently applied for identification of microbial invasion of the amniotic cavity. Our aim was to determine the levels of IL-6 in culture-positive amniotic fluids at genetic amniocentesis and to evaluate their significance in relation to pregnancy outcome. Methods. Seven culture-positive and 23 culture-negative amniotic fluids, obtained at 17-19 weeks of gestation, were analyzed for IL-6 levels by an immunoassay (ELISA). Pregnancy outcomes of all 30 women were obtained from the medical charts. Results. The mean level of IL-6 in the culture-negative amniotic fluids was 78 ± 206 pg/ml. Among the seven culture-positive, high levels of IL-6 were found only in three amniotic fluids that were culture-positive for Ureaplasma urealyticum (1834, 1342 and 2832 pg/ml). Low levels of IL-6, ranging from zero to 60 pg/ml, were found in four AFs that were culture-positive for Staphylococcus epidermidis (n = 3) and Bacillus Gram-positive (n = 1). Adverse pregnancy outcome occurred in the three women who had intra-amniotic infection with U. urealyticum accompanied by high levels of IL-6 (two fetal loss and one preterm delivery at 28 weeks of gestation). The four women with culture-positive but IL-6 negative amniotic fluids, had normal pregnancy outcome and term delivery. Two of the 23 women with culture-negative had preterm delivery, one with high (1000 pg/ml) and one with low (80 pg/ml) levels of IL-6. Conclusion. High levels of IL-6 are suggestive of a genuine intra-amniotic infection with urea-plasmas resulting in adverse pregnancy outcome, while culture-positive amniotic fluids with normal IL-6 levels, may suggest a state of contamination.

Original languageEnglish
Pages (from-to)379-382
Number of pages4
JournalActa Obstetricia et Gynecologica Scandinavica
Issue number5
StatePublished - 1 Jan 1999


  • Genetic amniocentesis
  • Interleukin-6
  • Intra-amniotic infection
  • Ureaplasma urealyticum

ASJC Scopus subject areas

  • Obstetrics and Gynecology


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