TY - JOUR
T1 - Elevated NADPH-oxidase activity in neutrophils from bile-duct-ligated rats
T2 - Changes in the kinetic parameters and in the oxidase cytosolic factor p47
AU - Levy, Rachel
AU - Nagauker, Ofra
AU - Sikuler, Emanuel
AU - Leto, Thomas L.
AU - Schlaeffer, Francisc
PY - 1994/2/17
Y1 - 1994/2/17
N2 - Stimulated superoxide generation was 2-fold higher in neutrophils from 20 rats with common bile-duct ligation (CBDL) compared to that of 20 sham-operated control rats. In order to study the mechanism of the higher NADPH oxidase activity in CBDL rats, the kinetic parameters of NADPH oxidase were analyzed. The Vmax of the NADPH oxidase in CBDL rat neutrophils was significantly higher than that of control rat neutrophils (10.2 and 5.3 nmol/min, respectively). The membrane and cytosol fractions of the oxidase were studied in a cell-free system. Neutrophil cytosol from CBDL rats added to neutrophil membranes from either CBDL or control rats produced 22.4 ± 1.6 and 21.0 ± 1.4 nmol/106 cells per 10 min, respectively. When neutrophil cytosol from control rats was mixed with neutrophil membranes from control or CBDL rats the generation of superoxide was 10.6 ± 1.4 and 10.0 ± 1.5 nmol/106 cells per 10 min, respectively. These results suggest that the cytosol components of the oxidase regulate its activity. By immunoblot analysis it was shown that the amount of the cytosolic factor p47 in neutrophils of CBDL rats is higher than that present in an equal number of neutrophils from control rats.
AB - Stimulated superoxide generation was 2-fold higher in neutrophils from 20 rats with common bile-duct ligation (CBDL) compared to that of 20 sham-operated control rats. In order to study the mechanism of the higher NADPH oxidase activity in CBDL rats, the kinetic parameters of NADPH oxidase were analyzed. The Vmax of the NADPH oxidase in CBDL rat neutrophils was significantly higher than that of control rat neutrophils (10.2 and 5.3 nmol/min, respectively). The membrane and cytosol fractions of the oxidase were studied in a cell-free system. Neutrophil cytosol from CBDL rats added to neutrophil membranes from either CBDL or control rats produced 22.4 ± 1.6 and 21.0 ± 1.4 nmol/106 cells per 10 min, respectively. When neutrophil cytosol from control rats was mixed with neutrophil membranes from control or CBDL rats the generation of superoxide was 10.6 ± 1.4 and 10.0 ± 1.5 nmol/106 cells per 10 min, respectively. These results suggest that the cytosol components of the oxidase regulate its activity. By immunoblot analysis it was shown that the amount of the cytosolic factor p47 in neutrophils of CBDL rats is higher than that present in an equal number of neutrophils from control rats.
KW - (Neutrophil)
KW - (Rat)
KW - Cell-free system
KW - Cytosolic factor p47
KW - NADPH oxidase activity
KW - Superoxide generation
UR - https://www.scopus.com/pages/publications/0028118688
U2 - 10.1016/0167-4889(94)90147-3
DO - 10.1016/0167-4889(94)90147-3
M3 - Article
AN - SCOPUS:0028118688
SN - 0167-4889
VL - 1220
SP - 261
EP - 265
JO - Biochimica et Biophysica Acta - Molecular Cell Research
JF - Biochimica et Biophysica Acta - Molecular Cell Research
IS - 3
ER -