Elimination of negative feedback control mechanisms along the insulin signaling pathway improves β-cell function under stress

Diana Gurevitch, Sigalit Boura-Halfon, Roi Isaac, Galit Shahaf, Moti Alberstein, Denise Ronen, Eli C. Lewis, Yehiel Zick

    Research output: Contribution to journalArticlepeer-review

    17 Scopus citations

    Abstract

    OBJECTIVE - Cellular stress and proinflammatory cytokines induce phosphorylation of insulin receptor substrate (IRS) proteins at Ser sites that inhibit insulin and IGF-1 signaling. Here, we examined the role of Ser phosphorylation of IRS-2 in mediating the inhibitory effects of proinflammatory cytokines and cellular stress on β-cell function. RESEARCH DESIGN AND METHODS - Five potential inhibitory Ser sites located proximally to the P-Tyr binding domain of IRS-2 were mutated to Ala. These IRS-2 mutants, denoted IRS-25A, and their wild-type controls (IRS-2WT) were introduced into adenoviral constructs that were infected into Min6 cells or into cultured murine islets. RESULTS - When expressed in cultured mouse islets, IRS-25A was better than IRS-2WT in protecting β-cells from apoptosis induced by a combination of IL-1β, IFN-γ, TNF-α, and Fas ligand. Cytokine-treated islets expressing IRS2 5A secreted significantly more insulin in response to glucose than did islets expressing IRS-2WT. This could be attributed to the higher transcription of Pdx1 in cytokine-treated islets that expressed IRS-2 5A. Accordingly, transplantation of 200 islets expressing IRS2 5A into STZ-induced diabetic mice restored their ability to respond to a glucose load similar to naïve mice. In contrast, mice transplanted with islets expressing IRS2WT maintained sustained hyperglycemia 3 days after transplantation. CONCLUSIONS - Elimination of a physiological negative feedback control mechanism along the insulin-signaling pathway that involves Ser/Thr phosphorylation of IRS-2 affords protection against the adverse effects of proinflammatory cytokines and improves β-cell function under stress. Genetic approaches that promote IRS25A expression in pancreatic β-cells, therefore, could be considered a rational treatment against β-cell failure after islet transplantation.

    Original languageEnglish
    Pages (from-to)2188-2197
    Number of pages10
    JournalDiabetes
    Volume59
    Issue number9
    DOIs
    StatePublished - 1 Jan 2010

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