TY - JOUR
T1 - Embracing Nature’s Wisdom
T2 - Liposome-Mediated Amplification of Electrochemiluminescence for the Sensitive and Selective Immunoassay of Serum Amyloid A
AU - Xu, Ruicheng
AU - Yang, Qiaoting
AU - Yang, Wei
AU - Zhang, Yuji
AU - Chauvin, Jérome
AU - Zhang, Xue Ji
AU - Cosnier, Serge
AU - Marks, Robert S.
AU - Shan, Dan
N1 - Publisher Copyright:
© 2024 American Chemical Society.
PY - 2025/1/14
Y1 - 2025/1/14
N2 - Serum amyloid A (SAA) is a key biomarker for diagnosing inflammatory responses in diseases like influenza and COVID-19. An electrochemiluminescence (ECL) biosensor has been constructed for signal enhancement in SAA detection by encapsulating 4,4′,4″,4‴-(1,3,6,8-pyrenetetrayl) tetrakis-benzoic acid (TBAPy) into liposomes. Such biomimetic encapsulation shields the biologically important membrane to avoid aggregation of TBAPy and prevents quenching. It would enhance the ECL signal’s stability and intensity, resulting in a 5-fold signal increase with a relative standard deviation (RSD) of 1.71%. The biosensor has shown very good specificity and sensitivity toward SAA detection. The estimated detection limit is 0.188 pg/mL. This innovative approach integrates biomimicry with ECL technology and represents a leap in developing the next generation of diagnostic tools with superior sensitivity and specificity.
AB - Serum amyloid A (SAA) is a key biomarker for diagnosing inflammatory responses in diseases like influenza and COVID-19. An electrochemiluminescence (ECL) biosensor has been constructed for signal enhancement in SAA detection by encapsulating 4,4′,4″,4‴-(1,3,6,8-pyrenetetrayl) tetrakis-benzoic acid (TBAPy) into liposomes. Such biomimetic encapsulation shields the biologically important membrane to avoid aggregation of TBAPy and prevents quenching. It would enhance the ECL signal’s stability and intensity, resulting in a 5-fold signal increase with a relative standard deviation (RSD) of 1.71%. The biosensor has shown very good specificity and sensitivity toward SAA detection. The estimated detection limit is 0.188 pg/mL. This innovative approach integrates biomimicry with ECL technology and represents a leap in developing the next generation of diagnostic tools with superior sensitivity and specificity.
UR - http://www.scopus.com/inward/record.url?scp=85214975594&partnerID=8YFLogxK
U2 - 10.1021/acs.analchem.4c05685
DO - 10.1021/acs.analchem.4c05685
M3 - Article
C2 - 39810342
AN - SCOPUS:85214975594
SN - 0003-2700
VL - 97
SP - 945
EP - 952
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 1
ER -