Endogenous, Ca2+-dependent cysteine-protease cleaves specifically the ryanodine receptor/Ca2+ release channel in skeletal muscle

V. Shoshan-Barmatz, S. Weil, H. Meyer, M. Varsanyi, L. M.G. Heilmeyer

Research output: Contribution to journalArticlepeer-review

38 Scopus citations


The association of an endogenous, Ca2+-dependent cysteine-protease with the junctional sarcoplasmic reticulum (SR) is demonstrated. The activity of this protease is strongly stimulated by dithiothreitol (DTT), cysteine and β-mercaptoethanol, and is inhibited by iodoacetamide, mercuric chloride and leupeptin, but not by PMSF. The activity of this thiol-protease is dependent on Ca2+ with half-maximal activity obtained at 0.1 μm and maximal activity at 10 μm. Mg2+ is also an activator of this enzyme (CI50=22 μm). These observations, together with the neutral pH optima and inhibition by the calpain I inhibitor, suggest that this enzyme is of calpain I type. This protease specifically cleaves the ryanodine receptor monomer (510 kD) at one site to produce two fragments with apparent molecular masses of 375 and 150 kD. The proteolytic fragments remain associated as shown by purification of the cleaved ryanodine receptor. The calpain binding site is identified as a PEST (proline, glutamic acid, serine, threonine-rich) region in the amino acid sequence GTPGGTPQPGVE, at positions 1356-1367 of the RyR and the cleavage site, the calmodulin binding site, at residues 1383-1400. The RyR cleavage by the Ca2+-dependent thiol-protease is prevented in the presence of ATP (1-5 m m) and by high NaCl concentrations. This cleavage of the RyR has no effect on ryanodine binding activity but stimulates Ca2+ efflux. A possible involvement of this specific cleavage of the RyR/Ca2+ release channel in the control of calpain activity is discussed.

Original languageEnglish
Pages (from-to)281-288
Number of pages8
JournalJournal of Membrane Biology
Issue number3
StatePublished - 1 Dec 1994


  • Ca release
  • Ca release channel
  • Calpain
  • Junctional sarcoplasmic reticulum
  • Ryanodine receptor


Dive into the research topics of 'Endogenous, Ca<sup>2+</sup>-dependent cysteine-protease cleaves specifically the ryanodine receptor/Ca<sup>2+</sup> release channel in skeletal muscle'. Together they form a unique fingerprint.

Cite this