Endowing plants with tolerance to virus infection by their preliminary treatment with short interfering RNAs

M. Yu Sutula, A. Zh Akbassova, T. M. Yergaliyev, Zh A. Nurbekova, G. S. Mukiyanova, R. T. Omarov

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

RNA interference (RNAi) is one of the key defense mechanisms directed against virus infections in plants and other organisms. In this case in plants infected with viruses, short interfering RNAs (siRNAs) are formed from two-chain replicated forms of virus molecules of RNA. These siRNAs program one of the RNAi basic components, RNA-induced complex of genes silencing (RISC, RNA induced silencing complex) associated with sequence-specific removing virus RNA. Virus protein P19 is a suppressor of RNAi and is capable of trapping the siRNAs being formed before their binding with RISC. Here, it was shown that preliminary entering leaves of plants Nicotiana benthamiana Domin (before virus infecting) of siRNAs eluted from the complex P19/siRNA from the infected plant lowers development of infection symptoms induced by tomato bushy stunt virus (TBSV) in inoculated plants. Exogenous addition of suppressor-associated siRNAs to plants leads to not only lowering virus accumulation but also to survival of infected plants. Thus, it has been established that preliminary addition of virus siRNAs elevates plant tolerance to the virus infection by means of early programming RISC and activation of the defense action of RNAi.

Original languageEnglish
Pages (from-to)939-945
Number of pages7
JournalRussian Journal of Plant Physiology
Volume64
Issue number6
DOIs
StatePublished - 1 Nov 2017
Externally publishedYes

Keywords

  • complex P19/siRNA
  • gel chromatography
  • Nicotiana benthamiana
  • RNA interference
  • siRNA

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