TY - JOUR
T1 - Enduring toxicity of transgenic Anabaena PCC 7120 expressing mosquito larvicidal genes from Bacillus thuringiensis ssp. israelensis
AU - Manasherob, Robert
AU - Otieno-Ayayo, Zachariah Ngalo
AU - Ben-Dov, Eitan
AU - Miaskovsky, Rina
AU - Boussiba, Sammy
AU - Zaritsky, Arieh
PY - 2003/10/1
Y1 - 2003/10/1
N2 - Persistence of biological control agents against mosquito larvae was tested under simulated field conditions. Mosquito larvicidal activity of transgenic Anabaena PCC 7120 expressing cry4Aa, cry11Aa and p20 from Bacillus thuringiensis ssp. israelensis was greater than B. thuringiensis ssp. israelensis primary powder (fun 89C06D) or wettable powder (WP) (Bactimos products) when either mixed with silt or exposed to sunlight outdoors. Reduction of Bactimos primary powder toxicity was at least 10-fold higher than Anabaena's after mixing with silt. In outdoors experiments, Bactimos WP remained toxic (over 30% mortality of 3rd instar Aedes aegypti larvae) for 2-4 days only, while transgenic Anabaena's toxicity endured 8-21 days.
AB - Persistence of biological control agents against mosquito larvae was tested under simulated field conditions. Mosquito larvicidal activity of transgenic Anabaena PCC 7120 expressing cry4Aa, cry11Aa and p20 from Bacillus thuringiensis ssp. israelensis was greater than B. thuringiensis ssp. israelensis primary powder (fun 89C06D) or wettable powder (WP) (Bactimos products) when either mixed with silt or exposed to sunlight outdoors. Reduction of Bactimos primary powder toxicity was at least 10-fold higher than Anabaena's after mixing with silt. In outdoors experiments, Bactimos WP remained toxic (over 30% mortality of 3rd instar Aedes aegypti larvae) for 2-4 days only, while transgenic Anabaena's toxicity endured 8-21 days.
UR - http://www.scopus.com/inward/record.url?scp=0141453317&partnerID=8YFLogxK
U2 - 10.1046/j.1462-2920.2003.00503.x
DO - 10.1046/j.1462-2920.2003.00503.x
M3 - Article
C2 - 14510853
AN - SCOPUS:0141453317
SN - 1462-2912
VL - 5
SP - 997
EP - 1001
JO - Environmental Microbiology
JF - Environmental Microbiology
IS - 10
ER -