Engineering Stem Cell Organoids

Xiaolei Yin; Benjamin E. Mead; Helia Safaee; Robert Langer; Jeffrey M. Karp; Oren Levy

doi:10.1016/j.stem.2015.12.005

Engineering Stem Cell Organoids

Xiaolei Yin
, Benjamin E. Mead
, Helia Safaee
, Robert Langer
, Jeffrey M. Karp
, Oren Levy

Research output: Contribution to journal › Review article › peer-review

773 Scopus citations

Abstract

Organoid systems leverage the self-organizing properties of stem cells to create diverse multi-cellular tissue proxies. Most organoid models only represent single or partial components of a tissue, and it is often difficult to control the cell type, organization, and cell-cell/cell-matrix interactions within these systems. Herein, we discuss basic approaches to generate stem cell-based organoids, their advantages and limitations, and how bioengineering strategies can be used to steer the cell composition and their 3D organization within organoids to further enhance their utility in research and therapies.

Original language	English
Pages (from-to)	25-38
Number of pages	14
Journal	Cell Stem Cell
Volume	18
Issue number	1
DOIs	https://doi.org/10.1016/j.stem.2015.12.005
State	Published - 7 Jan 2016
Externally published	Yes

ASJC Scopus subject areas

Molecular Medicine
Genetics
Cell Biology

Access to Document

10.1016/j.stem.2015.12.005

Cite this

@article{89bb7eecae9a4493924306ab75b5aa32,

title = "Engineering Stem Cell Organoids",

abstract = "Organoid systems leverage the self-organizing properties of stem cells to create diverse multi-cellular tissue proxies. Most organoid models only represent single or partial components of a tissue, and it is often difficult to control the cell type, organization, and cell-cell/cell-matrix interactions within these systems. Herein, we discuss basic approaches to generate stem cell-based organoids, their advantages and limitations, and how bioengineering strategies can be used to steer the cell composition and their 3D organization within organoids to further enhance their utility in research and therapies.",

author = "Xiaolei Yin and Mead, \{Benjamin E.\} and Helia Safaee and Robert Langer and Karp, \{Jeffrey M.\} and Oren Levy",

note = "Publisher Copyright: {\textcopyright} 2016 Elsevier Inc.",

year = "2016",

month = jan,

day = "7",

doi = "10.1016/j.stem.2015.12.005",

language = "English",

volume = "18",

pages = "25--38",

journal = "Cell Stem Cell",

issn = "1934-5909",

publisher = "Cell Press",

number = "1",

}

TY - JOUR

T1 - Engineering Stem Cell Organoids

AU - Yin, Xiaolei

AU - Mead, Benjamin E.

AU - Safaee, Helia

AU - Langer, Robert

AU - Karp, Jeffrey M.

AU - Levy, Oren

PY - 2016/1/7

Y1 - 2016/1/7

N2 - Organoid systems leverage the self-organizing properties of stem cells to create diverse multi-cellular tissue proxies. Most organoid models only represent single or partial components of a tissue, and it is often difficult to control the cell type, organization, and cell-cell/cell-matrix interactions within these systems. Herein, we discuss basic approaches to generate stem cell-based organoids, their advantages and limitations, and how bioengineering strategies can be used to steer the cell composition and their 3D organization within organoids to further enhance their utility in research and therapies.

AB - Organoid systems leverage the self-organizing properties of stem cells to create diverse multi-cellular tissue proxies. Most organoid models only represent single or partial components of a tissue, and it is often difficult to control the cell type, organization, and cell-cell/cell-matrix interactions within these systems. Herein, we discuss basic approaches to generate stem cell-based organoids, their advantages and limitations, and how bioengineering strategies can be used to steer the cell composition and their 3D organization within organoids to further enhance their utility in research and therapies.

UR - https://www.scopus.com/pages/publications/84954305673

U2 - 10.1016/j.stem.2015.12.005

DO - 10.1016/j.stem.2015.12.005

M3 - Review article

C2 - 26748754

AN - SCOPUS:84954305673

SN - 1934-5909

VL - 18

SP - 25

EP - 38

JO - Cell Stem Cell

JF - Cell Stem Cell

IS - 1

ER -

Engineering Stem Cell Organoids

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this