TY - JOUR
T1 - Enhanced bacterial affinity of PVDF membrane
T2 - its application as improved sea water sampling tool for environmental monitoring
AU - Kumar, Sweta Binod
AU - Sharnagat, Preeti
AU - Manna, Paramita
AU - Bhattacharya, Amit
AU - Haldar, Soumya
N1 - Funding Information:
SBK acknowledges DBT for financial support. We are also grateful to AD&CIF of CSIR-CSMCRI for analytical support. The manuscript has been assigned CSIR-CSMCRI-035/2016 registration number.
Publisher Copyright:
© 2017, Springer-Verlag Berlin Heidelberg.
PY - 2017/2/1
Y1 - 2017/2/1
N2 - Isolation of diversified bacteria from seawater is a major challenge in the field of environmental microbiology. In the present study, an attempt has been made to select specific membrane with improved property of attaching diversified bacteria. Initially, different concentrations (15, 18, and 20% W/W) of polysulfone (PSF) were used to check their affinity for the attachment of selected gram-positive (Bacillus subtilis) and gram-negative (Escherichia coli) bacteria. Among these, 20% W/W PSF showed maximum attachment. Therefore, membrane prepared with other materials such as polyvinylidene fluoride (PVDF) and polyether sulfone (PES) were used with the same concentration (20% W/W) to check their improved bacterial attachment property. Comparative study of bacterial attachment on three different membranes revealed that PVDF possessed the highest affinity towards both the groups of bacteria. This property was confirmed by different analytical methods viz. contact angle, atomic force microscopy, zeta potential, and flux study and further validated with seawater samples collected from seven sites of western coast and Lakshadweep island of India, using Biolog EcoPlate™. All the samples showed that bacterial richness and diversity was high in PVDF membrane in comparison to surrounding seawater samples. Interestingly, affinity for more diversified bacteria was reported to be higher in water sample with less turbidity and low bacteria load. This finding can facilitate the development of PVDF (20% W/W) membrane as a simple, cheap, and less labor intensive environmental sampling tool for the isolation of diversified bacteria from seawater sample wih different physiochemical properties. [Figure not available: see fulltext.]
AB - Isolation of diversified bacteria from seawater is a major challenge in the field of environmental microbiology. In the present study, an attempt has been made to select specific membrane with improved property of attaching diversified bacteria. Initially, different concentrations (15, 18, and 20% W/W) of polysulfone (PSF) were used to check their affinity for the attachment of selected gram-positive (Bacillus subtilis) and gram-negative (Escherichia coli) bacteria. Among these, 20% W/W PSF showed maximum attachment. Therefore, membrane prepared with other materials such as polyvinylidene fluoride (PVDF) and polyether sulfone (PES) were used with the same concentration (20% W/W) to check their improved bacterial attachment property. Comparative study of bacterial attachment on three different membranes revealed that PVDF possessed the highest affinity towards both the groups of bacteria. This property was confirmed by different analytical methods viz. contact angle, atomic force microscopy, zeta potential, and flux study and further validated with seawater samples collected from seven sites of western coast and Lakshadweep island of India, using Biolog EcoPlate™. All the samples showed that bacterial richness and diversity was high in PVDF membrane in comparison to surrounding seawater samples. Interestingly, affinity for more diversified bacteria was reported to be higher in water sample with less turbidity and low bacteria load. This finding can facilitate the development of PVDF (20% W/W) membrane as a simple, cheap, and less labor intensive environmental sampling tool for the isolation of diversified bacteria from seawater sample wih different physiochemical properties. [Figure not available: see fulltext.]
KW - Bacterial attachment
KW - Bacterial diversity
KW - Biolog EcoPlate™
KW - PVDF
KW - Sampling tool
KW - Seawater
UR - http://www.scopus.com/inward/record.url?scp=85008153159&partnerID=8YFLogxK
U2 - 10.1007/s11356-016-8318-1
DO - 10.1007/s11356-016-8318-1
M3 - Article
C2 - 28054272
AN - SCOPUS:85008153159
SN - 0944-1344
VL - 24
SP - 5831
EP - 5840
JO - Environmental Science and Pollution Research
JF - Environmental Science and Pollution Research
IS - 6
ER -