Enhancement of the rate of the β-elimination of phosphate from radicals derived from glycerol-2-phosphate by Cu(I)-phenanthroline. A pulse radiolysis study

Sara Goldstein, Gidon Czapski, Haim Cohen, Dan Meyerstein

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Hydroxyl radicals abstract hydrogen atoms from glycerol-2-phosphate with a specific rate constant of (7.0 ± 1.5) × 108 M-1s-1 forming the β-phospho radical as the major product. At physiological pH this radical undergoes a β-phosphate elimination with a rate constant ≤ 1 × 103 s-1. The β-phospho radical reacts with CU(I)-phenanthroline to produce an unstable transient with a metal-carbon σ-bond which has an absorbance similar to that of the cuprous phenanthroline complex in the visible region. This intermediate decomposes via a β-elimination of phosphate with a rate constant of 1.0 ± 1.5) × 104s-1, which was independent of the acidity in the pH range 4-9.

Original languageEnglish
Pages (from-to)371-379
Number of pages9
JournalFree Radical Biology and Medicine
Volume9
Issue number5
DOIs
StatePublished - 1 Jan 1990

Keywords

  • Aliphatic radical
  • Cu(I)-phenonanthroline
  • DNA damage
  • Free radicals
  • Radiation
  • β-elimination

ASJC Scopus subject areas

  • Biochemistry
  • Physiology (medical)

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