Enhancing the immunogenicity of liposomal hepatitis B surface antigen (HBsAg) by controlling its delivery from polymeric microspheres

Marcelle Machluf, Ron N. Apte, Oren Regev, Smadar Cohen

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

Microencapsulated liposome systems (MELs) were investigated as a potential immunization carrier for a recombinant 22-nm hepatitis B surface antigen (HBsAg) particle. MELs were prepared by first entrapping the HBsAg particles within liposomes composed of phosphatidylcholine:cholesterol (1:1 molar ratio), which were then encapsulated within alginate-poly(L-lysine) (PLL) hydrogel microspheres. The entrapped HBsAg particles retained immunoreactivity, as judged by an enzyme-linked immunosorbent assay (ELISA). Direct imaging of HBsAg particles and HBsAg incorporated into liposomes by cryo-transmission electron microscopy (cryo-TEM) indicated that HBsAg is embedded in the liposomal membrane. The antigenic particles were released from MELs mainly within the context of liposomes. The release rates in vitro and in vivo depended on the molecular weight of PLL used for MEL coating; MELs-214, coated with 214 kDa PLL, released the liposomal HBsAg at much higher rates than MELs-25, which was coated with 25 kDa PLL. Concomitantly, the specific anti-HBsAg titers in mice receiving HBsAg in MELs-214 were higher than those induced by MELs-25. MELs-214 were more efficient than conventional liposomes or alum in eliciting higher and prolonged antibody levels in mice. The ability of MELs to provide an HBsAg depot as well as a sustained release of liposomal HBsAg suggests that these carriers may be an ideal immunoadjuvant. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish
Pages (from-to)1550-1557
Number of pages8
JournalJournal of Pharmaceutical Sciences
Volume89
Issue number12
DOIs
StatePublished - 1 Jan 2000

Keywords

  • Cryo-transmission electron microscopy
  • Hepatitis B surface antigen
  • Humoral immune response
  • Liposomal antigens
  • Microencapsulated liposome systems
  • Slow release

ASJC Scopus subject areas

  • Pharmaceutical Science

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