TY - JOUR
T1 - Enrichment of rat tissue lipids with fatty acids that are prostaglandin precursors
AU - Danon, Abraham
AU - Heimberg, Murray
AU - Oates, John A.
N1 - Funding Information:
This work was supported in part by NIH Grants GMl5431, AM01677 and AM15498. We wish to thank Dr John Paulsrud, Hoffmann-La Roche Inc., Nutley, New Jersey, for Ro 3-1429/l and Dr John E. Pike, the Upjohn Company, Kalamazoo, Michigan, for the ethyl ester of 20 : 3~6. The capable technical assistance of Mrs Cynthia Dugger is acknowledged. The authors are particularly grateful to Dr John G. Coniglio for helpful advice and discussions.
PY - 1975/6/23
Y1 - 1975/6/23
N2 - 1. 1. The effects of supplementation of a complete diet with ethyl arachidonate and with ethyl dihomo-γ-linolenate (20 : 3ω6) on the fatty acid composition of plasma and tissue lipid classes were studied in normal rats. 2. 2. These prostaglandin precursors were incorporated in varying degrees into all lipid classes of the tissues that were investigated. The largest elevations were seen in plasma and tissue triacylglycerols. Significant increases were also observed in phospholipids, cholesteryl esters and the free fatty acid fraction. 3. 3. Following the feeding of the ester of 20 : 3ω6, arachidonate levels also rose in the lipids of some tissues. In others, such as the renal medulla and platelets, an increase in 20 : 3ω6 content occurred without a rise in 20 : 4. 4. 4. Platelet aggregation is known to be stimulated by 20 : 4 (via active metabolites), but not by 20 : 3ω6. The ability to modify 20 : 3ω6 levels selectively in certain tissues is of interest in light of such pharmacologic differences from 20 : 4.
AB - 1. 1. The effects of supplementation of a complete diet with ethyl arachidonate and with ethyl dihomo-γ-linolenate (20 : 3ω6) on the fatty acid composition of plasma and tissue lipid classes were studied in normal rats. 2. 2. These prostaglandin precursors were incorporated in varying degrees into all lipid classes of the tissues that were investigated. The largest elevations were seen in plasma and tissue triacylglycerols. Significant increases were also observed in phospholipids, cholesteryl esters and the free fatty acid fraction. 3. 3. Following the feeding of the ester of 20 : 3ω6, arachidonate levels also rose in the lipids of some tissues. In others, such as the renal medulla and platelets, an increase in 20 : 3ω6 content occurred without a rise in 20 : 4. 4. 4. Platelet aggregation is known to be stimulated by 20 : 4 (via active metabolites), but not by 20 : 3ω6. The ability to modify 20 : 3ω6 levels selectively in certain tissues is of interest in light of such pharmacologic differences from 20 : 4.
UR - http://www.scopus.com/inward/record.url?scp=0016740440&partnerID=8YFLogxK
U2 - 10.1016/0005-2760(75)90090-9
DO - 10.1016/0005-2760(75)90090-9
M3 - Article
AN - SCOPUS:0016740440
SN - 0005-2760
VL - 388
SP - 318
EP - 330
JO - Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
JF - Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
IS - 3
ER -