Enzymatic incorporation of an antibody-activated blue fluorophore into DNA

Gunnar F. Kaufmann; Michael M. Meijler; Chengzao Sun; Da Wei Chen; David P. Kujawa; Jenny M. Mee; Timothy Z. Hoffman; Peter Wirsching; Richard A. Lerner; Kim D. Janda

doi:10.1002/anie.200461143

Enzymatic incorporation of an antibody-activated blue fluorophore into DNA

Gunnar F. Kaufmann, Michael M. Meijler, Chengzao Sun, Da Wei Chen, David P. Kujawa, Jenny M. Mee, Timothy Z. Hoffman, Peter Wirsching, Richard A. Lerner, Kim D. Janda

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

(Chemical Equation Presented) Antibodies as light switches: The deoxy-nucleotide analogue-stilbene conjugate 1 can be incorporated into nascent DNA by DNA polymerase activity. The blue fluorescence of stilbene is detected only upon binding of an antibody that specifically recognizes the stilbene-modified nucleotide. Therefore, DNA generated through PCR with 1 in the substrate pool can be used for hybridization assays in which detection is triggered by the antibody (see scheme).

Original language	English
Pages (from-to)	2144-2148
Number of pages	5
Journal	Angewandte Chemie - International Edition
Volume	44
Issue number	14
DOIs	https://doi.org/10.1002/anie.200461143
State	Published - 29 Mar 2005
Externally published	Yes

Keywords

Antibodies
DNA recognition
Fluorescent probes
Nucleotides
Polymerase chain reaction

ASJC Scopus subject areas

Catalysis
Chemistry (all)

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10.1002/anie.200461143

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title = "Enzymatic incorporation of an antibody-activated blue fluorophore into DNA",

abstract = "(Chemical Equation Presented) Antibodies as light switches: The deoxy-nucleotide analogue-stilbene conjugate 1 can be incorporated into nascent DNA by DNA polymerase activity. The blue fluorescence of stilbene is detected only upon binding of an antibody that specifically recognizes the stilbene-modified nucleotide. Therefore, DNA generated through PCR with 1 in the substrate pool can be used for hybridization assays in which detection is triggered by the antibody (see scheme).",

keywords = "Antibodies, DNA recognition, Fluorescent probes, Nucleotides, Polymerase chain reaction",

author = "Kaufmann, {Gunnar F.} and Meijler, {Michael M.} and Chengzao Sun and Chen, {Da Wei} and Kujawa, {David P.} and Mee, {Jenny M.} and Hoffman, {Timothy Z.} and Peter Wirsching and Lerner, {Richard A.} and Janda, {Kim D.}",

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AU - Kaufmann, Gunnar F.

AU - Meijler, Michael M.

AU - Sun, Chengzao

AU - Chen, Da Wei

AU - Kujawa, David P.

AU - Mee, Jenny M.

AU - Hoffman, Timothy Z.

AU - Wirsching, Peter

AU - Lerner, Richard A.

AU - Janda, Kim D.

PY - 2005/3/29

Y1 - 2005/3/29

N2 - (Chemical Equation Presented) Antibodies as light switches: The deoxy-nucleotide analogue-stilbene conjugate 1 can be incorporated into nascent DNA by DNA polymerase activity. The blue fluorescence of stilbene is detected only upon binding of an antibody that specifically recognizes the stilbene-modified nucleotide. Therefore, DNA generated through PCR with 1 in the substrate pool can be used for hybridization assays in which detection is triggered by the antibody (see scheme).

AB - (Chemical Equation Presented) Antibodies as light switches: The deoxy-nucleotide analogue-stilbene conjugate 1 can be incorporated into nascent DNA by DNA polymerase activity. The blue fluorescence of stilbene is detected only upon binding of an antibody that specifically recognizes the stilbene-modified nucleotide. Therefore, DNA generated through PCR with 1 in the substrate pool can be used for hybridization assays in which detection is triggered by the antibody (see scheme).

KW - Antibodies

KW - DNA recognition

KW - Fluorescent probes

KW - Nucleotides

KW - Polymerase chain reaction

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JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

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ER -

Enzymatic incorporation of an antibody-activated blue fluorophore into DNA

Abstract

Keywords

ASJC Scopus subject areas

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