Enzymatic incorporation of an antibody-activated blue fluorophore into DNA

Gunnar F. Kaufmann, Michael M. Meijler, Chengzao Sun, Da Wei Chen, David P. Kujawa, Jenny M. Mee, Timothy Z. Hoffman, Peter Wirsching, Richard A. Lerner, Kim D. Janda

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

(Chemical Equation Presented) Antibodies as light switches: The deoxy-nucleotide analogue-stilbene conjugate 1 can be incorporated into nascent DNA by DNA polymerase activity. The blue fluorescence of stilbene is detected only upon binding of an antibody that specifically recognizes the stilbene-modified nucleotide. Therefore, DNA generated through PCR with 1 in the substrate pool can be used for hybridization assays in which detection is triggered by the antibody (see scheme).

Original languageEnglish
Pages (from-to)2144-2148
Number of pages5
JournalAngewandte Chemie - International Edition
Volume44
Issue number14
DOIs
StatePublished - 29 Mar 2005
Externally publishedYes

Keywords

  • Antibodies
  • DNA recognition
  • Fluorescent probes
  • Nucleotides
  • Polymerase chain reaction

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