Enzymatic synthesis of S-adenosyl-l-methionine from l-methionine and ATP

Akiva Gross; Shimona Geresh; George M. Whitesides

doi:10.1007/BF02779914

Enzymatic synthesis of S-adenosyl-l-methionine from l-methionine and ATP

Akiva Gross, Shimona Geresh, George M. Whitesides

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

S-Adenosyl-l-methionine (SAM) has been synthesized on 7-mmol scale from l-methionine and ATP. The ATP was generated in situ from AMP, and the synthesis of SAM was catalyzed by the enzyme ATP:l-methionine S-adenosyltransferase (EC 2.5.1.6). Although substantial effort was required to obtain even small quantities of enzyme, it has good stability once isolated and immobilized. The SAM produced by this procedure contains 89% of the (-) diasterioisomer and 11% of the (+) diasterioisomer.

Original language	English
Pages (from-to)	415-422
Number of pages	8
Journal	Applied Biochemistry and Biotechnology
Volume	8
Issue number	5
DOIs	https://doi.org/10.1007/BF02779914
State	Published - 1 Oct 1983
Externally published	Yes

Keywords

APT, generation from AMP
ATP:l-methionine S-adenosyl-transferase, immobilized
S-Adenosyl-l-methionine
catechol-O-methyl-transferase

ASJC Scopus subject areas

Biotechnology
Bioengineering
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology

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title = "Enzymatic synthesis of S-adenosyl-l-methionine from l-methionine and ATP",

abstract = "S-Adenosyl-l-methionine (SAM) has been synthesized on 7-mmol scale from l-methionine and ATP. The ATP was generated in situ from AMP, and the synthesis of SAM was catalyzed by the enzyme ATP:l-methionine S-adenosyltransferase (EC 2.5.1.6). Although substantial effort was required to obtain even small quantities of enzyme, it has good stability once isolated and immobilized. The SAM produced by this procedure contains 89% of the (-) diasterioisomer and 11% of the (+) diasterioisomer.",

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T1 - Enzymatic synthesis of S-adenosyl-l-methionine from l-methionine and ATP

AU - Gross, Akiva

AU - Geresh, Shimona

AU - Whitesides, George M.

PY - 1983/10/1

Y1 - 1983/10/1

N2 - S-Adenosyl-l-methionine (SAM) has been synthesized on 7-mmol scale from l-methionine and ATP. The ATP was generated in situ from AMP, and the synthesis of SAM was catalyzed by the enzyme ATP:l-methionine S-adenosyltransferase (EC 2.5.1.6). Although substantial effort was required to obtain even small quantities of enzyme, it has good stability once isolated and immobilized. The SAM produced by this procedure contains 89% of the (-) diasterioisomer and 11% of the (+) diasterioisomer.

AB - S-Adenosyl-l-methionine (SAM) has been synthesized on 7-mmol scale from l-methionine and ATP. The ATP was generated in situ from AMP, and the synthesis of SAM was catalyzed by the enzyme ATP:l-methionine S-adenosyltransferase (EC 2.5.1.6). Although substantial effort was required to obtain even small quantities of enzyme, it has good stability once isolated and immobilized. The SAM produced by this procedure contains 89% of the (-) diasterioisomer and 11% of the (+) diasterioisomer.

KW - APT, generation from AMP

KW - ATP:l-methionine S-adenosyl-transferase, immobilized

KW - S-Adenosyl-l-methionine

KW - catechol-O-methyl-transferase

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SN - 0273-2289

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EP - 422

JO - Applied Biochemistry and Biotechnology

JF - Applied Biochemistry and Biotechnology

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ER -

Enzymatic synthesis of S-adenosyl-l-methionine from l-methionine and ATP

Abstract

Keywords

ASJC Scopus subject areas

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