Enzyme-linked immunosorbent assay (ELISA) for detection of herpes simplex virus-specific IgM antibodies

Nina Kimmel, Maureen G. Friedman, Israel Sarov

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Herpes simplex virus (HSV)-specific IgM in human serum could be detected by a microplate enzyme-linked immunosorbent assay, using extracts of HSV-infected cells as antigen. Peroxidase-conjugated anti-human IgM was used to detect human IgM bound to viral antigen. Pretreatment of sera with protein A-bearing staphylococcus or with aggregated human IgG was necessary to eliminate false-positive results caused by the presence of rheumatoid factor. Specificity controls included sera of patients with other herpes group virus infections.

Original languageEnglish
Pages (from-to)219-227
Number of pages9
JournalJournal of Virological Methods
Volume4
Issue number4-5
DOIs
StatePublished - 1 Jan 1982
Externally publishedYes

Keywords

  • ELISA
  • IgM
  • herpes simplex virus

ASJC Scopus subject areas

  • Virology

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