TY - JOUR
T1 - Enzyme-linked immunosorbent assay (ELISA) for detection of herpes simplex virus-specific IgM antibodies
AU - Kimmel, Nina
AU - Friedman, Maureen G.
AU - Sarov, Israel
PY - 1982/1/1
Y1 - 1982/1/1
N2 - Herpes simplex virus (HSV)-specific IgM in human serum could be detected by a microplate enzyme-linked immunosorbent assay, using extracts of HSV-infected cells as antigen. Peroxidase-conjugated anti-human IgM was used to detect human IgM bound to viral antigen. Pretreatment of sera with protein A-bearing staphylococcus or with aggregated human IgG was necessary to eliminate false-positive results caused by the presence of rheumatoid factor. Specificity controls included sera of patients with other herpes group virus infections.
AB - Herpes simplex virus (HSV)-specific IgM in human serum could be detected by a microplate enzyme-linked immunosorbent assay, using extracts of HSV-infected cells as antigen. Peroxidase-conjugated anti-human IgM was used to detect human IgM bound to viral antigen. Pretreatment of sera with protein A-bearing staphylococcus or with aggregated human IgG was necessary to eliminate false-positive results caused by the presence of rheumatoid factor. Specificity controls included sera of patients with other herpes group virus infections.
KW - ELISA
KW - herpes simplex virus
KW - IgM
UR - http://www.scopus.com/inward/record.url?scp=0019956062&partnerID=8YFLogxK
U2 - 10.1016/0166-0934(82)90068-4
DO - 10.1016/0166-0934(82)90068-4
M3 - Article
C2 - 6286702
AN - SCOPUS:0019956062
SN - 0166-0934
VL - 4
SP - 219
EP - 227
JO - Journal of Virological Methods
JF - Journal of Virological Methods
IS - 4-5
ER -