Abstract
Herpes simplex virus (HSV)-specific IgM in human serum could be detected by a microplate enzyme-linked immunosorbent assay, using extracts of HSV-infected cells as antigen. Peroxidase-conjugated anti-human IgM was used to detect human IgM bound to viral antigen. Pretreatment of sera with protein A-bearing staphylococcus or with aggregated human IgG was necessary to eliminate false-positive results caused by the presence of rheumatoid factor. Specificity controls included sera of patients with other herpes group virus infections.
| Original language | English |
|---|---|
| Pages (from-to) | 219-227 |
| Number of pages | 9 |
| Journal | Journal of Virological Methods |
| Volume | 4 |
| Issue number | 4-5 |
| DOIs | |
| State | Published - 1 Jan 1982 |
Keywords
- ELISA
- IgM
- herpes simplex virus
ASJC Scopus subject areas
- Virology
Fingerprint
Dive into the research topics of 'Enzyme-linked immunosorbent assay (ELISA) for detection of herpes simplex virus-specific IgM antibodies'. Together they form a unique fingerprint.Cite this
- APA
- Author
- BIBTEX
- Harvard
- Standard
- RIS
- Vancouver