TY - JOUR
T1 - Enzymology of mammalian phospholipases D
T2 - in vitro studies
AU - Liscovitch, Mordechai
AU - Chalifa-Caspi, Vered
N1 - Funding Information:
We thank Yona Eli for excellentte chnicaal ssis-tance.S omeof the work carried out in our laboratory was supported by grants from the German-IsraelFi oundationf or Researcha nd Development( GIF) and the Israel ScienceF ounda-tion.
PY - 1996/5/24
Y1 - 1996/5/24
N2 - The existence of multiple forms of phopholipase D was clearly established in a large number of biochemical studies that described and characterized the enzymological properties of the different PLD activities. This review summarizes the in vitro evidence showing differential subcellular localization and chromatographic properties of putative PLD isozymes, their phospholipid and alcohol substrate specificities, their modulation by various divalent cations, small G proteins and protein kinase c isozymes, and the role of phosphatidylinositol 4,5-bisphosphate as a cofactor of phospholipase D.
AB - The existence of multiple forms of phopholipase D was clearly established in a large number of biochemical studies that described and characterized the enzymological properties of the different PLD activities. This review summarizes the in vitro evidence showing differential subcellular localization and chromatographic properties of putative PLD isozymes, their phospholipid and alcohol substrate specificities, their modulation by various divalent cations, small G proteins and protein kinase c isozymes, and the role of phosphatidylinositol 4,5-bisphosphate as a cofactor of phospholipase D.
KW - Enzymology
KW - In vitro studies
KW - Isoenzymes
KW - Phospholipase D
UR - http://www.scopus.com/inward/record.url?scp=0029990785&partnerID=8YFLogxK
U2 - 10.1016/0009-3084(96)02544-3
DO - 10.1016/0009-3084(96)02544-3
M3 - Article
AN - SCOPUS:0029990785
SN - 0009-3084
VL - 80
SP - 37
EP - 44
JO - Chemistry and Physics of Lipids
JF - Chemistry and Physics of Lipids
IS - 1-2
ER -