Epitaxial sandwiching of biopolymers

D. G. Brandon; M. Talianker; L. Gal-or; Y. Komem

doi:10.1016/0039-6028(78)90431-4

Epitaxial sandwiching of biopolymers

D. G. Brandon, M. Talianker, L. Gal-or, Y. Komem

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

A technique has been developed for occluding biopolymer molecules in thin films of epitaxial gold. The method relies on deposition of the biopolymer from aqueous solution by "streaking" followed by continued ordered deposition of gold on the single crystal gold support film. The advantage of this technique of specimen preparation for electron microscopy are the high resolution (of the order of 0.5 nm) and excellent stability while under observation. Good contrast has been obtained reproducibly and is associated with the presence of the biopolymer as an occluded defect in the gold crystal.

Original language	English
Pages (from-to)	566-577
Number of pages	12
Journal	Surface Science
Volume	70
Issue number	1
DOIs	https://doi.org/10.1016/0039-6028(78)90431-4
State	Published - 1 Jan 1978
Externally published	Yes

ASJC Scopus subject areas

Condensed Matter Physics
Surfaces and Interfaces
Surfaces, Coatings and Films
Materials Chemistry

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10.1016/0039-6028(78)90431-4

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title = "Epitaxial sandwiching of biopolymers",

abstract = "A technique has been developed for occluding biopolymer molecules in thin films of epitaxial gold. The method relies on deposition of the biopolymer from aqueous solution by {"}streaking{"} followed by continued ordered deposition of gold on the single crystal gold support film. The advantage of this technique of specimen preparation for electron microscopy are the high resolution (of the order of 0.5 nm) and excellent stability while under observation. Good contrast has been obtained reproducibly and is associated with the presence of the biopolymer as an occluded defect in the gold crystal.",

author = "Brandon, {D. G.} and M. Talianker and L. Gal-or and Y. Komem",

note = "Funding Information: The authors would like to thank Larissa Patlajan for her help in the early stages of this work. They would also like to acknowledget he financial support provided by the Israel Academy of Sciencesa nd the Humanities,a nd the National Institutes of Health (Grant No. 5 ROl GM22264-02 MBY). Finally, the authorsm ust express their debt of gratitudet o their many friends and colleaguesw ho have providedb oth practical advicea nd activeh elp in ail stageso f the project, especiallyH annah Shalitin (Dept. of Biology, Technion) and Bob Josephs ~eizmann Institute, Rellovoth).",

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doi = "10.1016/0039-6028(78)90431-4",

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T1 - Epitaxial sandwiching of biopolymers

AU - Brandon, D. G.

AU - Talianker, M.

AU - Gal-or, L.

AU - Komem, Y.

N1 - Funding Information: The authors would like to thank Larissa Patlajan for her help in the early stages of this work. They would also like to acknowledget he financial support provided by the Israel Academy of Sciencesa nd the Humanities,a nd the National Institutes of Health (Grant No. 5 ROl GM22264-02 MBY). Finally, the authorsm ust express their debt of gratitudet o their many friends and colleaguesw ho have providedb oth practical advicea nd activeh elp in ail stageso f the project, especiallyH annah Shalitin (Dept. of Biology, Technion) and Bob Josephs ~eizmann Institute, Rellovoth).

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N2 - A technique has been developed for occluding biopolymer molecules in thin films of epitaxial gold. The method relies on deposition of the biopolymer from aqueous solution by "streaking" followed by continued ordered deposition of gold on the single crystal gold support film. The advantage of this technique of specimen preparation for electron microscopy are the high resolution (of the order of 0.5 nm) and excellent stability while under observation. Good contrast has been obtained reproducibly and is associated with the presence of the biopolymer as an occluded defect in the gold crystal.

AB - A technique has been developed for occluding biopolymer molecules in thin films of epitaxial gold. The method relies on deposition of the biopolymer from aqueous solution by "streaking" followed by continued ordered deposition of gold on the single crystal gold support film. The advantage of this technique of specimen preparation for electron microscopy are the high resolution (of the order of 0.5 nm) and excellent stability while under observation. Good contrast has been obtained reproducibly and is associated with the presence of the biopolymer as an occluded defect in the gold crystal.

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U2 - 10.1016/0039-6028(78)90431-4

DO - 10.1016/0039-6028(78)90431-4

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EP - 577

JO - Surface Science

JF - Surface Science

SN - 0039-6028

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ER -

Epitaxial sandwiching of biopolymers

Abstract

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