Essential role of TNF-α in gamma c cytokine aided crosstalk between dendritic cells and natural killer cells in experimental murine lymphoma

Uttam Gupta; Sumit Kumar Hira; Ranjeet Singh; Ankush Paladhi; Prateek Srivastava; Partha Pratim Manna

doi:10.1016/j.intimp.2019.106031

Essential role of TNF-α in gamma c cytokine aided crosstalk between dendritic cells and natural killer cells in experimental murine lymphoma

Uttam Gupta, Sumit Kumar Hira, Ranjeet Singh, Ankush Paladhi, Prateek Srivastava, Partha Pratim Manna

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Cooperative and cognitive interaction between the dendritic cells and natural killer cells was investigated for demonstrating the anti-tumor activity against an aggressive murine lymphoma, treated with doxorubicin. Crosstalk between the dendritic cells and the natural killer cells significantly reduced the proliferation of Dalton's lymphoma cells in a dose dependent manner. Treatment of Dalton's lymphoma cells with doxorubicin in vitro enhances the effects of crosstalk against the target cells. This crosstalk between the cells was regulated via stimulation with recombinant interleukin-15, and release of TNF-α which is critically important for the tumoricidal effects. Dendritic cells and the natural killer cells crosstalk activate both the cells and upregulate the expression of CD40, CD69 and CD86 on the dendritic cells. These findings provided new insight regarding these interactions and define a mechanism by which cellular immune response promotes tumoricidal activity against lymphoma in therapeutic setting.

Original language	English
Article number	106031
Journal	International Immunopharmacology
Volume	78
DOIs	https://doi.org/10.1016/j.intimp.2019.106031
State	Published - 1 Jan 2020
Externally published	Yes

Keywords

Cross-talk
Cytotoxicity
Dendritic cells
Doxorubicin
Growth inhibition
Natural killer cells
TNF-α

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Pharmacology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.intimp.2019.106031

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title = "Essential role of TNF-α in gamma c cytokine aided crosstalk between dendritic cells and natural killer cells in experimental murine lymphoma",

abstract = "Cooperative and cognitive interaction between the dendritic cells and natural killer cells was investigated for demonstrating the anti-tumor activity against an aggressive murine lymphoma, treated with doxorubicin. Crosstalk between the dendritic cells and the natural killer cells significantly reduced the proliferation of Dalton's lymphoma cells in a dose dependent manner. Treatment of Dalton's lymphoma cells with doxorubicin in vitro enhances the effects of crosstalk against the target cells. This crosstalk between the cells was regulated via stimulation with recombinant interleukin-15, and release of TNF-α which is critically important for the tumoricidal effects. Dendritic cells and the natural killer cells crosstalk activate both the cells and upregulate the expression of CD40, CD69 and CD86 on the dendritic cells. These findings provided new insight regarding these interactions and define a mechanism by which cellular immune response promotes tumoricidal activity against lymphoma in therapeutic setting.",

keywords = "Cross-talk, Cytotoxicity, Dendritic cells, Doxorubicin, Growth inhibition, Natural killer cells, TNF-α",

author = "Uttam Gupta and Hira, {Sumit Kumar} and Ranjeet Singh and Ankush Paladhi and Prateek Srivastava and {Pratim Manna}, Partha",

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year = "2020",

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doi = "10.1016/j.intimp.2019.106031",

language = "English",

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T1 - Essential role of TNF-α in gamma c cytokine aided crosstalk between dendritic cells and natural killer cells in experimental murine lymphoma

AU - Gupta, Uttam

AU - Hira, Sumit Kumar

AU - Singh, Ranjeet

AU - Paladhi, Ankush

AU - Srivastava, Prateek

AU - Pratim Manna, Partha

PY - 2020/1/1

Y1 - 2020/1/1

N2 - Cooperative and cognitive interaction between the dendritic cells and natural killer cells was investigated for demonstrating the anti-tumor activity against an aggressive murine lymphoma, treated with doxorubicin. Crosstalk between the dendritic cells and the natural killer cells significantly reduced the proliferation of Dalton's lymphoma cells in a dose dependent manner. Treatment of Dalton's lymphoma cells with doxorubicin in vitro enhances the effects of crosstalk against the target cells. This crosstalk between the cells was regulated via stimulation with recombinant interleukin-15, and release of TNF-α which is critically important for the tumoricidal effects. Dendritic cells and the natural killer cells crosstalk activate both the cells and upregulate the expression of CD40, CD69 and CD86 on the dendritic cells. These findings provided new insight regarding these interactions and define a mechanism by which cellular immune response promotes tumoricidal activity against lymphoma in therapeutic setting.

AB - Cooperative and cognitive interaction between the dendritic cells and natural killer cells was investigated for demonstrating the anti-tumor activity against an aggressive murine lymphoma, treated with doxorubicin. Crosstalk between the dendritic cells and the natural killer cells significantly reduced the proliferation of Dalton's lymphoma cells in a dose dependent manner. Treatment of Dalton's lymphoma cells with doxorubicin in vitro enhances the effects of crosstalk against the target cells. This crosstalk between the cells was regulated via stimulation with recombinant interleukin-15, and release of TNF-α which is critically important for the tumoricidal effects. Dendritic cells and the natural killer cells crosstalk activate both the cells and upregulate the expression of CD40, CD69 and CD86 on the dendritic cells. These findings provided new insight regarding these interactions and define a mechanism by which cellular immune response promotes tumoricidal activity against lymphoma in therapeutic setting.

KW - Cross-talk

KW - Cytotoxicity

KW - Dendritic cells

KW - Doxorubicin

KW - Growth inhibition

KW - Natural killer cells

KW - TNF-α

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DO - 10.1016/j.intimp.2019.106031

M3 - Article

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AN - SCOPUS:85076017746

SN - 1567-5769

VL - 78

JO - International Immunopharmacology

JF - International Immunopharmacology

M1 - 106031

ER -

Essential role of TNF-α in gamma c cytokine aided crosstalk between dendritic cells and natural killer cells in experimental murine lymphoma

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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