Ethylene formation in Pisum sativum and Vicia faba protoplasts

Micha Guy, Hans Kende

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

Protoplasts isolated from leaves of peas (Pisum sativum L.) and of Vicia faba L. produced 1-aminocyclopropane-1-carboxylic acid (ACC) from endogenous substrate. Synthesis of ACC and conversion of ACC to ethylene was promoted by light and inhibited by 3-(3,4-dichlorophenyl)-1,1-dimethylurea and carbonyl cyanide m-chlorophenylhydrazone. Aminoethoxyvinylglycine inhibited ethylene synthesis to a minor extent when given during incubation of the protoplasts but was very effective when added both to the medium in which the protoplasts were isolated and to the incubation medium as well. Radioactivity from [U-14C]methionine was incorporated into ACC and ethylene. However, the specific radioactivity of the C-2 and C-3 atoms of ACC, from which ethylene is formed, increased much faster than the specific radioactivity of ethylene. It appears that ACC and ethylene are synthesized in different compartments of the cell and that protoplasts constitute a suitable system to study this compartmentation.

Original languageEnglish
Pages (from-to)276-280
Number of pages5
JournalPlanta
Volume160
Issue number3
DOIs
StatePublished - 1 Mar 1984
Externally publishedYes

Keywords

  • 1-Aminocyclopropane-1-carboxylic acid
  • Aminoethoxyvinylglycine
  • Ethylene synthesis
  • Pisum (ethylene synthesis)
  • Vicia

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