Evaluation of microbial colony growth parameters by laser speckle imaging

Ilya Balmages, Janis Liepins, Ernests T. Auzins, Anitra Zile, Dmitrijs Bliznuks, Ilze Lihacova, Alexey Lihachev

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

3 Scopus citations

Abstract

Our previous studies have shown that it is possible to determine the growth activity of a microbial colony by laser speckle imaging techniques. A sub-pixel correlation method was proposed to detect small changes in the sequence of laser speckle images. In this study, we compared the laser speckle imaging method to the reference method - image time series under white light to detect the colony growth parameters (growth rate, critical detection time).

Original languageEnglish
Title of host publicationBiomedical Spectroscopy, Microscopy, and Imaging II
EditorsJurgen Popp, Csilla Gergely
PublisherSPIE
ISBN (Electronic)9781510651647
DOIs
StatePublished - 1 Jan 2022
Externally publishedYes
EventBiomedical Spectroscopy, Microscopy, and Imaging II 2022 - Virtual, Online
Duration: 9 May 202220 May 2022

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume12144
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X

Conference

ConferenceBiomedical Spectroscopy, Microscopy, and Imaging II 2022
CityVirtual, Online
Period9/05/2220/05/22

Keywords

  • image processing
  • laser speckle imaging
  • microbial colony forming units
  • microorganism activity estimation
  • sensitive sub-pixel correlation method

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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