Abstract
High-throughput screening (HTS) of enzymatic activity is important for directed evolution-based enzyme engineering. However, substrate and product diffusion can severely compromise these HTS assays. In this issue of Chemistry & Biology, Kintses and coworkers describe a microfluidic platform for the directed evolution of enzymes in droplets that allows for the screening of 10 7 mutants per round of evolution.
Original language | English |
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Pages (from-to) | 929-931 |
Number of pages | 3 |
Journal | Chemistry and Biology |
Volume | 19 |
Issue number | 8 |
DOIs | |
State | Published - 24 Aug 2012 |
ASJC Scopus subject areas
- Biochemistry
- Molecular Medicine
- Molecular Biology
- Pharmacology
- Drug Discovery
- Clinical Biochemistry