Evolution in microfluidic droplet

Itay Levin; Amir Aharoni

doi:10.1016/j.chembiol.2012.08.004

Evolution in microfluidic droplet

Itay Levin, Amir Aharoni

Department of Life Sciences

Research output: Contribution to journal › Short survey › peer-review

12 Scopus citations

Abstract

High-throughput screening (HTS) of enzymatic activity is important for directed evolution-based enzyme engineering. However, substrate and product diffusion can severely compromise these HTS assays. In this issue of Chemistry & Biology, Kintses and coworkers describe a microfluidic platform for the directed evolution of enzymes in droplets that allows for the screening of 10 ⁷ mutants per round of evolution.

Original language	English
Pages (from-to)	929-931
Number of pages	3
Journal	Chemistry and Biology
Volume	19
Issue number	8
DOIs	https://doi.org/10.1016/j.chembiol.2012.08.004
State	Published - 24 Aug 2012

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Pharmacology
Drug Discovery
Clinical Biochemistry

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title = "Evolution in microfluidic droplet",

abstract = " High-throughput screening (HTS) of enzymatic activity is important for directed evolution-based enzyme engineering. However, substrate and product diffusion can severely compromise these HTS assays. In this issue of Chemistry \& Biology, Kintses and coworkers describe a microfluidic platform for the directed evolution of enzymes in droplets that allows for the screening of 10 7 mutants per round of evolution.",

author = "Itay Levin and Amir Aharoni",

year = "2012",

month = aug,

day = "24",

doi = "10.1016/j.chembiol.2012.08.004",

language = "English",

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journal = "Chemistry and Biology",

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TY - JOUR

T1 - Evolution in microfluidic droplet

AU - Levin, Itay

AU - Aharoni, Amir

PY - 2012/8/24

Y1 - 2012/8/24

N2 - High-throughput screening (HTS) of enzymatic activity is important for directed evolution-based enzyme engineering. However, substrate and product diffusion can severely compromise these HTS assays. In this issue of Chemistry & Biology, Kintses and coworkers describe a microfluidic platform for the directed evolution of enzymes in droplets that allows for the screening of 10 7 mutants per round of evolution.

AB - High-throughput screening (HTS) of enzymatic activity is important for directed evolution-based enzyme engineering. However, substrate and product diffusion can severely compromise these HTS assays. In this issue of Chemistry & Biology, Kintses and coworkers describe a microfluidic platform for the directed evolution of enzymes in droplets that allows for the screening of 10 7 mutants per round of evolution.

UR - https://www.scopus.com/pages/publications/84865457748

U2 - 10.1016/j.chembiol.2012.08.004

DO - 10.1016/j.chembiol.2012.08.004

M3 - Short survey

AN - SCOPUS:84865457748

SN - 1074-5521

VL - 19

SP - 929

EP - 931

JO - Chemistry and Biology

JF - Chemistry and Biology

IS - 8

ER -

Evolution in microfluidic droplet

Abstract

ASJC Scopus subject areas

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