Evolution of interorganismal strigolactone biosynthesis in seed plants

Anqi Zhou; Annalise Kane; Sheng Wu; Kaibiao Wang; Michell Santiago; Yui Ishiguro; Kaori Yoneyama; Malathy Palayam; Nitzan Shabek; Xiaonan Xie; David C. Nelson; Yanran Li

doi:10.1126/science.adp0779

Evolution of interorganismal strigolactone biosynthesis in seed plants

Anqi Zhou
, Annalise Kane
, Sheng Wu
, Kaibiao Wang
, Michell Santiago
, Yui Ishiguro
, Kaori Yoneyama
, Malathy Palayam
, Nitzan Shabek
, Xiaonan Xie
, David C. Nelson
, Yanran Li

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

Strigolactones (SLs) are methylbutenolide molecules derived from b-carotene through an intermediate carlactonoic acid (CLA). Canonical SLs act as signals to microbes and plants, whereas noncanonical SLs are primarily plant hormones. The cytochrome P450 CYP722C catalyzes a critical step, converting CLA to canonical SLs in most angiosperms. Using synthetic biology, we investigated the function of CYP722A, an evolutionary predecessor of CYP722C. CYP722A converts CLA into 16-hydroxy-CLA (16-OH-CLA), a noncanonical SL detected exclusively in the shoots of various flowering plants. 16-OH-CLA application restores control of shoot branching to SL-deficient mutants in Arabidopsis thaliana and is perceived by the SL signaling pathway. We hypothesize that biosynthesis of 16-OH-CLA by CYP722A was a metabolic stepping stone in the evolution of canonical SLs that mediate rhizospheric signaling in many flowering plants.

Original language	English
Article number	eadp0779
Journal	Science
Volume	387
Issue number	6731
DOIs	https://doi.org/10.1126/science.adp0779
State	Published - 17 Jan 2025
Externally published	Yes

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title = "Evolution of interorganismal strigolactone biosynthesis in seed plants",

abstract = "Strigolactones (SLs) are methylbutenolide molecules derived from b-carotene through an intermediate carlactonoic acid (CLA). Canonical SLs act as signals to microbes and plants, whereas noncanonical SLs are primarily plant hormones. The cytochrome P450 CYP722C catalyzes a critical step, converting CLA to canonical SLs in most angiosperms. Using synthetic biology, we investigated the function of CYP722A, an evolutionary predecessor of CYP722C. CYP722A converts CLA into 16-hydroxy-CLA (16-OH-CLA), a noncanonical SL detected exclusively in the shoots of various flowering plants. 16-OH-CLA application restores control of shoot branching to SL-deficient mutants in Arabidopsis thaliana and is perceived by the SL signaling pathway. We hypothesize that biosynthesis of 16-OH-CLA by CYP722A was a metabolic stepping stone in the evolution of canonical SLs that mediate rhizospheric signaling in many flowering plants.",

author = "Anqi Zhou and Annalise Kane and Sheng Wu and Kaibiao Wang and Michell Santiago and Yui Ishiguro and Kaori Yoneyama and Malathy Palayam and Nitzan Shabek and Xiaonan Xie and Nelson, \{David C.\} and Yanran Li",

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doi = "10.1126/science.adp0779",

language = "English",

volume = "387",

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TY - JOUR

T1 - Evolution of interorganismal strigolactone biosynthesis in seed plants

AU - Zhou, Anqi

AU - Kane, Annalise

AU - Wu, Sheng

AU - Wang, Kaibiao

AU - Santiago, Michell

AU - Ishiguro, Yui

AU - Yoneyama, Kaori

AU - Palayam, Malathy

AU - Shabek, Nitzan

AU - Xie, Xiaonan

AU - Nelson, David C.

AU - Li, Yanran

PY - 2025/1/17

Y1 - 2025/1/17

N2 - Strigolactones (SLs) are methylbutenolide molecules derived from b-carotene through an intermediate carlactonoic acid (CLA). Canonical SLs act as signals to microbes and plants, whereas noncanonical SLs are primarily plant hormones. The cytochrome P450 CYP722C catalyzes a critical step, converting CLA to canonical SLs in most angiosperms. Using synthetic biology, we investigated the function of CYP722A, an evolutionary predecessor of CYP722C. CYP722A converts CLA into 16-hydroxy-CLA (16-OH-CLA), a noncanonical SL detected exclusively in the shoots of various flowering plants. 16-OH-CLA application restores control of shoot branching to SL-deficient mutants in Arabidopsis thaliana and is perceived by the SL signaling pathway. We hypothesize that biosynthesis of 16-OH-CLA by CYP722A was a metabolic stepping stone in the evolution of canonical SLs that mediate rhizospheric signaling in many flowering plants.

AB - Strigolactones (SLs) are methylbutenolide molecules derived from b-carotene through an intermediate carlactonoic acid (CLA). Canonical SLs act as signals to microbes and plants, whereas noncanonical SLs are primarily plant hormones. The cytochrome P450 CYP722C catalyzes a critical step, converting CLA to canonical SLs in most angiosperms. Using synthetic biology, we investigated the function of CYP722A, an evolutionary predecessor of CYP722C. CYP722A converts CLA into 16-hydroxy-CLA (16-OH-CLA), a noncanonical SL detected exclusively in the shoots of various flowering plants. 16-OH-CLA application restores control of shoot branching to SL-deficient mutants in Arabidopsis thaliana and is perceived by the SL signaling pathway. We hypothesize that biosynthesis of 16-OH-CLA by CYP722A was a metabolic stepping stone in the evolution of canonical SLs that mediate rhizospheric signaling in many flowering plants.

UR - https://www.scopus.com/pages/publications/85216064347

U2 - 10.1126/science.adp0779

DO - 10.1126/science.adp0779

M3 - Article

C2 - 39818909

AN - SCOPUS:85216064347

SN - 0036-8075

VL - 387

JO - Science

JF - Science

IS - 6731

M1 - eadp0779

ER -

Evolution of interorganismal strigolactone biosynthesis in seed plants

Abstract

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