TY - BOOK
T1 - Evolutionary Origin of the Mammalian Hematopoietic System Found in a Colonial Chordate
AU - Rosental, Benyamin
AU - Mark, B. U.N.
AU - Kowarsky, Mark
AU - Seita, Jun
AU - Corey, Daniel
AU - Ishizuka, Katherine
AU - Palmeri, Karla
AU - Chen, Shih-Yu
AU - Sinha, Rahul
AU - Okamoto, Jennifer
AU - Mantalas, Gary
AU - Manni, Lucia
AU - Raveh, Tal
AU - Clarke, Nat
AU - Newman, Aaron
AU - Neff, Norma
AU - Nolan, Garry
AU - Quake, Stephen
AU - Weissman, Irving
AU - Voskoboynik, Ayelet
PY - 2017/12/26
Y1 - 2017/12/26
N2 - Hematopoiesis is an essential process that evolved in multicellular animals. At the heart of this process are hematopoietic stem cells (HSCs), which are multipotent, self-renewing and generate the entire repertoire of blood and immune cells throughout life. Here we studied the hematopoietic system of Botryllus schlosseri , a colonial tunicate that has vasculature, circulating blood cells, and interesting characteristics of stem cell biology and immunity. Self-recognition between genetically compatible B. schlosseri colonies leads to the formation of natural parabionts with shared circulation, whereas incompatible colonies reject each other. Using flow-cytometry, whole-transcriptome sequencing of defined cell populations, and diverse functional assays, we identified HSCs, progenitors, immune-effector cells, the HSC niche, and demonstrated that self-recognition inhibits cytotoxic reaction. Our study implies that the HSC and myeloid lineages emerged in a common ancestor of tunicates and vertebrates and suggests that hematopoietic bone marrow and the B. schlosseri endostyle niche evolved from the same origin.
AB - Hematopoiesis is an essential process that evolved in multicellular animals. At the heart of this process are hematopoietic stem cells (HSCs), which are multipotent, self-renewing and generate the entire repertoire of blood and immune cells throughout life. Here we studied the hematopoietic system of Botryllus schlosseri , a colonial tunicate that has vasculature, circulating blood cells, and interesting characteristics of stem cell biology and immunity. Self-recognition between genetically compatible B. schlosseri colonies leads to the formation of natural parabionts with shared circulation, whereas incompatible colonies reject each other. Using flow-cytometry, whole-transcriptome sequencing of defined cell populations, and diverse functional assays, we identified HSCs, progenitors, immune-effector cells, the HSC niche, and demonstrated that self-recognition inhibits cytotoxic reaction. Our study implies that the HSC and myeloid lineages emerged in a common ancestor of tunicates and vertebrates and suggests that hematopoietic bone marrow and the B. schlosseri endostyle niche evolved from the same origin.
U2 - 10.1101/206318
DO - 10.1101/206318
M3 - Book
BT - Evolutionary Origin of the Mammalian Hematopoietic System Found in a Colonial Chordate
ER -