Experimental proof of concept of nanoparticle-assisted STED

Yannick Sonnefraud; Hugo G. Sinclair; Yonatan Sivan; Matthew R. Foreman; Christopher W. Dunsby; Mark A.A. Neil; Paul M. French; Stefan A. Maier

doi:10.1021/nl5014103

Experimental proof of concept of nanoparticle-assisted STED

Yannick Sonnefraud, Hugo G. Sinclair, Yonatan Sivan, Matthew R. Foreman, Christopher W. Dunsby, Mark A.A. Neil, Paul M. French, Stefan A. Maier

Unit of Photonics and Electro-Optics Engineering

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

We imaged core-shell nanoparticles, consisting of a dye-doped silica core covered with a layer of gold, with a stimulated emission depletion, fluorescence lifetime imaging (STED-FLIM) microscope. Because of the field enhancement provided by the localized surface plasmon resonance of the gold shell, we demonstrate a reduction of the STED depletion power required to obtain resolution improvement by a factor of 4. This validates the concept of nanoparticle-assisted STED (NP-STED), where hybrid dye-plasmonic nanoparticles are used as labels for STED in order to decrease the depletion powers required for subwavelength imaging.

Original language	English
Pages (from-to)	4449-4453
Number of pages	5
Journal	Nano Letters
Volume	14
Issue number	8
DOIs	https://doi.org/10.1021/nl5014103
State	Published - 13 Aug 2014

Keywords

Plasmonics
STED microscopy
fluorescence
microscopy
nanoshells

ASJC Scopus subject areas

Bioengineering
Chemistry (all)
Materials Science (all)
Condensed Matter Physics
Mechanical Engineering

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10.1021/nl5014103

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title = "Experimental proof of concept of nanoparticle-assisted STED",

abstract = "We imaged core-shell nanoparticles, consisting of a dye-doped silica core covered with a layer of gold, with a stimulated emission depletion, fluorescence lifetime imaging (STED-FLIM) microscope. Because of the field enhancement provided by the localized surface plasmon resonance of the gold shell, we demonstrate a reduction of the STED depletion power required to obtain resolution improvement by a factor of 4. This validates the concept of nanoparticle-assisted STED (NP-STED), where hybrid dye-plasmonic nanoparticles are used as labels for STED in order to decrease the depletion powers required for subwavelength imaging.",

keywords = "Plasmonics, STED microscopy, fluorescence, microscopy, nanoshells",

author = "Yannick Sonnefraud and Sinclair, {Hugo G.} and Yonatan Sivan and Foreman, {Matthew R.} and Dunsby, {Christopher W.} and Neil, {Mark A.A.} and French, {Paul M.} and Maier, {Stefan A.}",

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doi = "10.1021/nl5014103",

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T1 - Experimental proof of concept of nanoparticle-assisted STED

AU - Sonnefraud, Yannick

AU - Sinclair, Hugo G.

AU - Sivan, Yonatan

AU - Foreman, Matthew R.

AU - Dunsby, Christopher W.

AU - Neil, Mark A.A.

AU - French, Paul M.

AU - Maier, Stefan A.

PY - 2014/8/13

Y1 - 2014/8/13

N2 - We imaged core-shell nanoparticles, consisting of a dye-doped silica core covered with a layer of gold, with a stimulated emission depletion, fluorescence lifetime imaging (STED-FLIM) microscope. Because of the field enhancement provided by the localized surface plasmon resonance of the gold shell, we demonstrate a reduction of the STED depletion power required to obtain resolution improvement by a factor of 4. This validates the concept of nanoparticle-assisted STED (NP-STED), where hybrid dye-plasmonic nanoparticles are used as labels for STED in order to decrease the depletion powers required for subwavelength imaging.

AB - We imaged core-shell nanoparticles, consisting of a dye-doped silica core covered with a layer of gold, with a stimulated emission depletion, fluorescence lifetime imaging (STED-FLIM) microscope. Because of the field enhancement provided by the localized surface plasmon resonance of the gold shell, we demonstrate a reduction of the STED depletion power required to obtain resolution improvement by a factor of 4. This validates the concept of nanoparticle-assisted STED (NP-STED), where hybrid dye-plasmonic nanoparticles are used as labels for STED in order to decrease the depletion powers required for subwavelength imaging.

KW - Plasmonics

KW - STED microscopy

KW - fluorescence

KW - microscopy

KW - nanoshells

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SP - 4449

EP - 4453

JO - Nano Letters

JF - Nano Letters

IS - 8

ER -

Experimental proof of concept of nanoparticle-assisted STED

Abstract

Keywords

ASJC Scopus subject areas

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