Exploitation of microbes for enhancing bacoside content and reduction of Meloidogyne incognita infestation in Bacopa monnieri L

Rupali Gupta, Sudeep Tiwari, Shilpi K. Saikia, Virendra Shukla, Rashmi Singh, S. P. Singh, P. V.Ajay Kumar, Rakesh Pandey

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Despite the vast exploration of rhizospheric microbial wealth for crop yield enhancement, knowledge about the efficacy of microbial agents as biocontrol weapons against root-knot disease is scarce, especially in medicinal plants, viz., Bacopa monnieri. In the present investigation, rhizospheric microbes, viz., Bacillus megaterium, Glomus intraradices, Trichoderma harzianum ThU, and their combinations were evaluated for the management of Meloidogyne incognita (Kofoid and White) Chitwood and bacoside content enhancement in B. monnieri var CIM-Jagriti. A novel validated method Fourier transform near infrared was used for rapid estimation of total bacoside content. A significant reduction (2.75-fold) in root-knot indices was observed in the combined treatment of B. megaterium and T. harzianum ThU in comparison to untreated control plants. The same treatment also showed significant enhancement (1.40-fold) in total bacoside contents (plant active molecule) content using Fourier transform near-infrared (FT-NIR) method that analyses samples rapidly in an hour without solvent usage and provides ample scope for natural product studies.

Original languageEnglish
Pages (from-to)53-61
Number of pages9
JournalProtoplasma
Volume252
Issue number1
DOIs
StatePublished - 1 Jan 2015
Externally publishedYes

Keywords

  • Asparginase activity
  • FT-NIR
  • Indole acetic acid
  • Larvicidal
  • Meloidogyne incognita
  • Root-knot index

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

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