Exploring the potential of CRISPR/Cas genome editing for vegetable crop improvement: An overview of challenges and approaches

Tuyelee Das, Uttpal Anand, Tarun Pal, Sayanti Mandal, Manoj Kumar, Radha, Abilash Valsala Gopalakrishnan, José M.Pérez de la Lastra, Abhijit Dey

Research output: Contribution to journalReview articlepeer-review

7 Scopus citations

Abstract

Vegetables provide many nutrients in the form of fiber, vitamins, and minerals, which make them an important part of our diet. Numerous biotic and abiotic stresses can affect crop growth, quality, and yield. Traditional and modern breeding strategies to improve plant traits are slow and resource intensive. Therefore, it is necessary to find new approaches for crop improvement. Clustered regularly interspaced short palindromic repeats/CRISPR associated 9 (CRISPR/Cas9) is a genome editing tool that can be used to modify targeted genes for desirable traits with greater efficiency and accuracy. By using CRISPR/Cas9 editing to precisely mutate key genes, it is possible to rapidly generate new germplasm resources for the promotion of important agronomic traits. This is made possible by the availability of whole genome sequencing data and information on the function of genes responsible for important traits. In addition, CRISPR/Cas9 systems have revolutionized agriculture, making genome editing more versatile. Currently, genome editing of vegetable crops is limited to a few vegetable varieties (tomato, sweet potato, potato, carrot, squash, eggplant, etc.) due to lack of regeneration protocols and sufficient genome sequencing data. In this article, we summarize recent studies on the application of CRISPR/Cas9 in improving vegetable trait development and the potential for future improvement.

Original languageEnglish
Pages (from-to)1215-1228
Number of pages14
JournalBiotechnology and Bioengineering
Volume120
Issue number5
DOIs
StatePublished - 1 May 2023

Keywords

  • CRISPR/Cas9
  • gene knockout
  • regulatory framework
  • stress tolerance
  • trait improvement
  • vegetable breeding

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Bioengineering
  • Biotechnology

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