Exposure to naphthalene and β-pinene-derived secondary organic aerosol induced divergent changes in transcript levels of BEAS-2B cells

Michal Pardo; Svenja Offer; Elena Hartner; Sebastiano Di Bucchianico; Christoph Bisig; Stefanie Bauer; Jana Pantzke; Elias J. Zimmermann; Xin Cao; Stephanie Binder; Evelyn Kuhn; Anja Huber; Seongho Jeong; Uwe Käfer; Eric Schneider; Arunas Mesceriakovas; Jan Bendl; Ramona Brejcha; Angela Buchholz; Daniela Gat; Thorsten Hohaus; Narges Rastak; Erwin Karg; Gert Jakobi; Markus Kalberer; Tamara Kanashova; Yue Hu; Christoph Ogris; Annalisa Marsico; Fabian Theis; Tali Shalit; Thomas Gröger; Christopher P. Rüger; Sebastian Oeder; Jürgen Orasche; Andreas Paul; Till Ziehm; Zhi Hui Zhang; Thomas Adam; Olli Sippula; Martin Sklorz; Jürgen Schnelle-Kreis; Hendryk Czech; Astrid Kiendler-Scharr; Ralf Zimmermann; Yinon Rudich

doi:10.1016/j.envint.2022.107366

Exposure to naphthalene and β-pinene-derived secondary organic aerosol induced divergent changes in transcript levels of BEAS-2B cells

Michal Pardo, Svenja Offer, Elena Hartner, Sebastiano Di Bucchianico, Christoph Bisig, Stefanie Bauer, Jana Pantzke, Elias J. Zimmermann, Xin Cao, Stephanie Binder, Evelyn Kuhn, Anja Huber, Seongho Jeong, Uwe Käfer, Eric Schneider, Arunas Mesceriakovas, Jan Bendl, Ramona Brejcha, Angela Buchholz, Daniela GatThorsten Hohaus, Narges Rastak, Erwin Karg, Gert Jakobi, Markus Kalberer, Tamara Kanashova, Yue Hu, Christoph Ogris, Annalisa Marsico, Fabian Theis, Tali Shalit, Thomas Gröger, Christopher P. Rüger, Sebastian Oeder, Jürgen Orasche, Andreas Paul, Till Ziehm, Zhi Hui Zhang, Thomas Adam, Olli Sippula, Martin Sklorz, Jürgen Schnelle-Kreis, Hendryk Czech, Astrid Kiendler-Scharr, Ralf Zimmermann, Yinon Rudich

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

The health effects of exposure to secondary organic aerosols (SOAs) are still limited. Here, we investigated and compared the toxicities of soot particles (SP) coated with β-pinene SOA (SOA_βPin-SP) and SP coated with naphthalene SOA (SOA_Nap-SP) in a human bronchial epithelial cell line (BEAS-2B) residing at the air–liquid interface. SOA_βPin-SP mostly contained oxygenated aliphatic compounds from β-pinene photooxidation, whereas SOA_Nap-SP contained a significant fraction of oxygenated aromatic products under similar conditions. Following exposure, genome-wide transcriptome responses showed an Nrf2 oxidative stress response, particularly for SOA_Nap-SP. Other signaling pathways, such as redox signaling, inflammatory signaling, and the involvement of matrix metalloproteinase, were identified to have a stronger impact following exposure to SOA_Nap-SP. SOA_Nap-SP also induced a stronger genotoxicity response than that of SOA_βPin-SP. This study elucidated the mechanisms that govern SOA toxicity and showed that, compared to SOAs derived from a typical biogenic precursor, SOAs from a typical anthropogenic precursor have higher toxicological potency, which was accompanied with the activation of varied cellular mechanisms, such as aryl hydrocarbon receptor. This can be attributed to the difference in chemical composition; specifically, the aromatic compounds in the naphthalene-derived SOA had higher cytotoxic potential than that of the β-pinene-derived SOA.

Original language	English
Article number	107366
Journal	Environment international
Volume	166
DOIs	https://doi.org/10.1016/j.envint.2022.107366
State	Published - 1 Aug 2022
Externally published	Yes

Keywords

Cytotoxicity
Health effects
Particulate matter
RNA sequencing
Signaling pathway
Soot particles

ASJC Scopus subject areas

General Environmental Science

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.envint.2022.107366

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Pardo, M., Offer, S., Hartner, E., Di Bucchianico, S., Bisig, C., Bauer, S., Pantzke, J., Zimmermann, E. J., Cao, X., Binder, S., Kuhn, E., Huber, A., Jeong, S., Käfer, U., Schneider, E., Mesceriakovas, A., Bendl, J., Brejcha, R., Buchholz, A., ... Rudich, Y. (2022). Exposure to naphthalene and β-pinene-derived secondary organic aerosol induced divergent changes in transcript levels of BEAS-2B cells. Environment international, 166, Article 107366. https://doi.org/10.1016/j.envint.2022.107366

@article{1d733b5781334343b19579e0e40c1255,

title = "Exposure to naphthalene and β-pinene-derived secondary organic aerosol induced divergent changes in transcript levels of BEAS-2B cells",

abstract = "The health effects of exposure to secondary organic aerosols (SOAs) are still limited. Here, we investigated and compared the toxicities of soot particles (SP) coated with β-pinene SOA (SOAβPin-SP) and SP coated with naphthalene SOA (SOANap-SP) in a human bronchial epithelial cell line (BEAS-2B) residing at the air–liquid interface. SOAβPin-SP mostly contained oxygenated aliphatic compounds from β-pinene photooxidation, whereas SOANap-SP contained a significant fraction of oxygenated aromatic products under similar conditions. Following exposure, genome-wide transcriptome responses showed an Nrf2 oxidative stress response, particularly for SOANap-SP. Other signaling pathways, such as redox signaling, inflammatory signaling, and the involvement of matrix metalloproteinase, were identified to have a stronger impact following exposure to SOANap-SP. SOANap-SP also induced a stronger genotoxicity response than that of SOAβPin-SP. This study elucidated the mechanisms that govern SOA toxicity and showed that, compared to SOAs derived from a typical biogenic precursor, SOAs from a typical anthropogenic precursor have higher toxicological potency, which was accompanied with the activation of varied cellular mechanisms, such as aryl hydrocarbon receptor. This can be attributed to the difference in chemical composition; specifically, the aromatic compounds in the naphthalene-derived SOA had higher cytotoxic potential than that of the β-pinene-derived SOA.",

keywords = "Cytotoxicity, Health effects, Particulate matter, RNA sequencing, Signaling pathway, Soot particles",

author = "Michal Pardo and Svenja Offer and Elena Hartner and {Di Bucchianico}, Sebastiano and Christoph Bisig and Stefanie Bauer and Jana Pantzke and Zimmermann, {Elias J.} and Xin Cao and Stephanie Binder and Evelyn Kuhn and Anja Huber and Seongho Jeong and Uwe K{\"a}fer and Eric Schneider and Arunas Mesceriakovas and Jan Bendl and Ramona Brejcha and Angela Buchholz and Daniela Gat and Thorsten Hohaus and Narges Rastak and Erwin Karg and Gert Jakobi and Markus Kalberer and Tamara Kanashova and Yue Hu and Christoph Ogris and Annalisa Marsico and Fabian Theis and Tali Shalit and Thomas Gr{\"o}ger and R{\"u}ger, {Christopher P.} and Sebastian Oeder and J{\"u}rgen Orasche and Andreas Paul and Till Ziehm and Zhang, {Zhi Hui} and Thomas Adam and Olli Sippula and Martin Sklorz and J{\"u}rgen Schnelle-Kreis and Hendryk Czech and Astrid Kiendler-Scharr and Ralf Zimmermann and Yinon Rudich",

note = "Publisher Copyright: {\textcopyright} 2022",

year = "2022",

month = aug,

day = "1",

doi = "10.1016/j.envint.2022.107366",

language = "English",

volume = "166",

journal = "Environment international",

issn = "0160-4120",

publisher = "Elsevier Ltd.",

}

Pardo, M, Offer, S, Hartner, E, Di Bucchianico, S, Bisig, C, Bauer, S, Pantzke, J, Zimmermann, EJ, Cao, X, Binder, S, Kuhn, E, Huber, A, Jeong, S, Käfer, U, Schneider, E, Mesceriakovas, A, Bendl, J, Brejcha, R, Buchholz, A, Gat, D, Hohaus, T, Rastak, N, Karg, E, Jakobi, G, Kalberer, M, Kanashova, T, Hu, Y, Ogris, C, Marsico, A, Theis, F, Shalit, T, Gröger, T, Rüger, CP, Oeder, S, Orasche, J, Paul, A, Ziehm, T, Zhang, ZH, Adam, T, Sippula, O, Sklorz, M, Schnelle-Kreis, J, Czech, H, Kiendler-Scharr, A, Zimmermann, R & Rudich, Y 2022, 'Exposure to naphthalene and β-pinene-derived secondary organic aerosol induced divergent changes in transcript levels of BEAS-2B cells', Environment international, vol. 166, 107366. https://doi.org/10.1016/j.envint.2022.107366

TY - JOUR

T1 - Exposure to naphthalene and β-pinene-derived secondary organic aerosol induced divergent changes in transcript levels of BEAS-2B cells

AU - Pardo, Michal

AU - Offer, Svenja

AU - Hartner, Elena

AU - Di Bucchianico, Sebastiano

AU - Bisig, Christoph

AU - Bauer, Stefanie

AU - Pantzke, Jana

AU - Zimmermann, Elias J.

AU - Cao, Xin

AU - Binder, Stephanie

AU - Kuhn, Evelyn

AU - Huber, Anja

AU - Jeong, Seongho

AU - Käfer, Uwe

AU - Schneider, Eric

AU - Mesceriakovas, Arunas

AU - Bendl, Jan

AU - Brejcha, Ramona

AU - Buchholz, Angela

AU - Gat, Daniela

AU - Hohaus, Thorsten

AU - Rastak, Narges

AU - Karg, Erwin

AU - Jakobi, Gert

AU - Kalberer, Markus

AU - Kanashova, Tamara

AU - Hu, Yue

AU - Ogris, Christoph

AU - Marsico, Annalisa

AU - Theis, Fabian

AU - Shalit, Tali

AU - Gröger, Thomas

AU - Rüger, Christopher P.

AU - Oeder, Sebastian

AU - Orasche, Jürgen

AU - Paul, Andreas

AU - Ziehm, Till

AU - Zhang, Zhi Hui

AU - Adam, Thomas

AU - Sippula, Olli

AU - Sklorz, Martin

AU - Schnelle-Kreis, Jürgen

AU - Czech, Hendryk

AU - Kiendler-Scharr, Astrid

AU - Zimmermann, Ralf

AU - Rudich, Yinon

PY - 2022/8/1

Y1 - 2022/8/1

N2 - The health effects of exposure to secondary organic aerosols (SOAs) are still limited. Here, we investigated and compared the toxicities of soot particles (SP) coated with β-pinene SOA (SOAβPin-SP) and SP coated with naphthalene SOA (SOANap-SP) in a human bronchial epithelial cell line (BEAS-2B) residing at the air–liquid interface. SOAβPin-SP mostly contained oxygenated aliphatic compounds from β-pinene photooxidation, whereas SOANap-SP contained a significant fraction of oxygenated aromatic products under similar conditions. Following exposure, genome-wide transcriptome responses showed an Nrf2 oxidative stress response, particularly for SOANap-SP. Other signaling pathways, such as redox signaling, inflammatory signaling, and the involvement of matrix metalloproteinase, were identified to have a stronger impact following exposure to SOANap-SP. SOANap-SP also induced a stronger genotoxicity response than that of SOAβPin-SP. This study elucidated the mechanisms that govern SOA toxicity and showed that, compared to SOAs derived from a typical biogenic precursor, SOAs from a typical anthropogenic precursor have higher toxicological potency, which was accompanied with the activation of varied cellular mechanisms, such as aryl hydrocarbon receptor. This can be attributed to the difference in chemical composition; specifically, the aromatic compounds in the naphthalene-derived SOA had higher cytotoxic potential than that of the β-pinene-derived SOA.

AB - The health effects of exposure to secondary organic aerosols (SOAs) are still limited. Here, we investigated and compared the toxicities of soot particles (SP) coated with β-pinene SOA (SOAβPin-SP) and SP coated with naphthalene SOA (SOANap-SP) in a human bronchial epithelial cell line (BEAS-2B) residing at the air–liquid interface. SOAβPin-SP mostly contained oxygenated aliphatic compounds from β-pinene photooxidation, whereas SOANap-SP contained a significant fraction of oxygenated aromatic products under similar conditions. Following exposure, genome-wide transcriptome responses showed an Nrf2 oxidative stress response, particularly for SOANap-SP. Other signaling pathways, such as redox signaling, inflammatory signaling, and the involvement of matrix metalloproteinase, were identified to have a stronger impact following exposure to SOANap-SP. SOANap-SP also induced a stronger genotoxicity response than that of SOAβPin-SP. This study elucidated the mechanisms that govern SOA toxicity and showed that, compared to SOAs derived from a typical biogenic precursor, SOAs from a typical anthropogenic precursor have higher toxicological potency, which was accompanied with the activation of varied cellular mechanisms, such as aryl hydrocarbon receptor. This can be attributed to the difference in chemical composition; specifically, the aromatic compounds in the naphthalene-derived SOA had higher cytotoxic potential than that of the β-pinene-derived SOA.

KW - Cytotoxicity

KW - Health effects

KW - Particulate matter

KW - RNA sequencing

KW - Signaling pathway

KW - Soot particles

UR - http://www.scopus.com/inward/record.url?scp=85133808113&partnerID=8YFLogxK

U2 - 10.1016/j.envint.2022.107366

DO - 10.1016/j.envint.2022.107366

M3 - Article

C2 - 35763991

AN - SCOPUS:85133808113

SN - 0160-4120

VL - 166

JO - Environment international

JF - Environment international

M1 - 107366

ER -

Exposure to naphthalene and β-pinene-derived secondary organic aerosol induced divergent changes in transcript levels of BEAS-2B cells

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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