Expressed sequence tag analysis of khat (Catha edulis) provides a putative molecular biochemical basis for the biosynthesis of phenylpropylamino alkaloids

Jillian M. Hagel; Raz Krizevski; Korey Kilpatrick; Yaron Sitrit; Frédéric Marsolais; Efraim Lewinsohn; Peter J. Facchini

doi:10.1590/S1415-47572011000400017

Expressed sequence tag analysis of khat (Catha edulis) provides a putative molecular biochemical basis for the biosynthesis of phenylpropylamino alkaloids

Jillian M. Hagel, Raz Krizevski, Korey Kilpatrick, Yaron Sitrit, Frédéric Marsolais, Efraim Lewinsohn, Peter J. Facchini

The French Associates Institute for Agriculture and Biotechnology of Drylands

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Khat (Catha edulisForsk.) is a flowering perennial shrub cultivated for its neurostimulant properties resulting mainly from the occurrence of (S)-cathinone in young leaves. The biosynthesis of (S)-cathinone and the related phenyl-propylamino alkaloids (1S,2S)-cathine and (1R, 2S)-norephedrine is not well characterized in plants. We prepared a cDNA library from young khat leaves and sequenced 4,896 random clones, generating an expressed sequence tag (EST) library of 3,293 unigenes. Putative functions were assigned to > 98% of the ESTs, providing a key resource for gene discovery. Candidates potentially involved at various stages of phenylpropylamino alkaloid biosynthesis from L-phenylalanine to (1S,2S)-cathine were identified.

Original language	English
Pages (from-to)	640-646
Number of pages	7
Journal	Genetics and Molecular Biology
Volume	34
Issue number	4
DOIs	https://doi.org/10.1590/S1415-47572011000400017
State	Published - 1 Jan 2011

Keywords

(S)-cathinone
EST library
Gene discovery
Khat
Phenylpropylamino alkaloids biosynthesis

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1590/S1415-47572011000400017

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title = "Expressed sequence tag analysis of khat (Catha edulis) provides a putative molecular biochemical basis for the biosynthesis of phenylpropylamino alkaloids",

abstract = "Khat (Catha edulisForsk.) is a flowering perennial shrub cultivated for its neurostimulant properties resulting mainly from the occurrence of (S)-cathinone in young leaves. The biosynthesis of (S)-cathinone and the related phenyl-propylamino alkaloids (1S,2S)-cathine and (1R, 2S)-norephedrine is not well characterized in plants. We prepared a cDNA library from young khat leaves and sequenced 4,896 random clones, generating an expressed sequence tag (EST) library of 3,293 unigenes. Putative functions were assigned to > 98% of the ESTs, providing a key resource for gene discovery. Candidates potentially involved at various stages of phenylpropylamino alkaloid biosynthesis from L-phenylalanine to (1S,2S)-cathine were identified.",

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AU - Hagel, Jillian M.

AU - Krizevski, Raz

AU - Kilpatrick, Korey

AU - Sitrit, Yaron

AU - Marsolais, Frédéric

AU - Lewinsohn, Efraim

AU - Facchini, Peter J.

PY - 2011/1/1

Y1 - 2011/1/1

N2 - Khat (Catha edulisForsk.) is a flowering perennial shrub cultivated for its neurostimulant properties resulting mainly from the occurrence of (S)-cathinone in young leaves. The biosynthesis of (S)-cathinone and the related phenyl-propylamino alkaloids (1S,2S)-cathine and (1R, 2S)-norephedrine is not well characterized in plants. We prepared a cDNA library from young khat leaves and sequenced 4,896 random clones, generating an expressed sequence tag (EST) library of 3,293 unigenes. Putative functions were assigned to > 98% of the ESTs, providing a key resource for gene discovery. Candidates potentially involved at various stages of phenylpropylamino alkaloid biosynthesis from L-phenylalanine to (1S,2S)-cathine were identified.

AB - Khat (Catha edulisForsk.) is a flowering perennial shrub cultivated for its neurostimulant properties resulting mainly from the occurrence of (S)-cathinone in young leaves. The biosynthesis of (S)-cathinone and the related phenyl-propylamino alkaloids (1S,2S)-cathine and (1R, 2S)-norephedrine is not well characterized in plants. We prepared a cDNA library from young khat leaves and sequenced 4,896 random clones, generating an expressed sequence tag (EST) library of 3,293 unigenes. Putative functions were assigned to > 98% of the ESTs, providing a key resource for gene discovery. Candidates potentially involved at various stages of phenylpropylamino alkaloid biosynthesis from L-phenylalanine to (1S,2S)-cathine were identified.

KW - (S)-cathinone

KW - EST library

KW - Gene discovery

KW - Khat

KW - Phenylpropylamino alkaloids biosynthesis

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DO - 10.1590/S1415-47572011000400017

M3 - Article

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SN - 1415-4757

VL - 34

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JO - Genetics and Molecular Biology

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ER -

Expressed sequence tag analysis of khat (Catha edulis) provides a putative molecular biochemical basis for the biosynthesis of phenylpropylamino alkaloids

Abstract

Keywords

ASJC Scopus subject areas

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