TY - JOUR
T1 - Expression and localization of muscarinic receptors in P19-derived neurons
AU - Parnas, Dorit
AU - Heldman, Eliahu
AU - Branski, Leora
AU - Feinstein, Naomi
AU - Linial, Michal
N1 - Funding Information:
We would like to thank A. I. Levey (Emory University, Atlanta) for the generous gifts of antibodies. We also thank the Smith laboratory in the Hebrew University for the use of their facilities. M. L. is a member of the Otto Lewie Minerva Center for molecular and cellular neurobiology in Jerusalem. This work was partially supported by the National Institute for Psychobiology in Israel.
PY - 1998/1/1
Y1 - 1998/1/1
N2 - The muscarinic acetylcholine receptors are important in a variety of physiological processes such as induction of secretion from various glands and regulation of pacemaker activity, muscle tone, and neurotransmission. To date, the muscarinic receptor family includes five members (designated m1- m5), of which m1-m4 are abundant in brain and in peripheral tissues, and m5 is found exclusively in brain, and even there at very low levels. The expression of m1-m5 receptor subtypes was studied in neurons derived from the murine embryonal carcinoma cell line P19. These cells serve as a model system for differentiation and maturation of neurons resembling CNS neurons. Our results show that P19 neurons express mainly the m2, m3, and m5 subtypes. Low levels of m1 receptors are also detected and m4 subtype is practically absent. Furthermore, muscarinic receptors in P19 neurons are functional in activating second messenger signaling pathways. The localization of m2 receptors is predominantly presynaptic, whereas the m5 subtype is mainly postsynaptic. Consequently, P19 cells provide a model system for the study of pre- and postsynaptic muscarinic acetylcholine-receptor subtypes in a proper neuronal context. This is particularly valid for the rare m5 receptors.
AB - The muscarinic acetylcholine receptors are important in a variety of physiological processes such as induction of secretion from various glands and regulation of pacemaker activity, muscle tone, and neurotransmission. To date, the muscarinic receptor family includes five members (designated m1- m5), of which m1-m4 are abundant in brain and in peripheral tissues, and m5 is found exclusively in brain, and even there at very low levels. The expression of m1-m5 receptor subtypes was studied in neurons derived from the murine embryonal carcinoma cell line P19. These cells serve as a model system for differentiation and maturation of neurons resembling CNS neurons. Our results show that P19 neurons express mainly the m2, m3, and m5 subtypes. Low levels of m1 receptors are also detected and m4 subtype is practically absent. Furthermore, muscarinic receptors in P19 neurons are functional in activating second messenger signaling pathways. The localization of m2 receptors is predominantly presynaptic, whereas the m5 subtype is mainly postsynaptic. Consequently, P19 cells provide a model system for the study of pre- and postsynaptic muscarinic acetylcholine-receptor subtypes in a proper neuronal context. This is particularly valid for the rare m5 receptors.
KW - Adenylyl cyclase
KW - Embryonal carcinoma
KW - Inositol (1,4,5)- trisphosphate
KW - Neuronal cell-line
KW - Presynaptic receptors
UR - http://www.scopus.com/inward/record.url?scp=0031841711&partnerID=8YFLogxK
U2 - 10.1007/BF02737082
DO - 10.1007/BF02737082
M3 - Article
C2 - 9589367
AN - SCOPUS:0031841711
SN - 0895-8696
VL - 10
SP - 17
EP - 29
JO - Journal of Molecular Neuroscience
JF - Journal of Molecular Neuroscience
IS - 1
ER -
