Expression in Escherichia coli of the native cyt1Aa from Bacillus thuringiensis subsp. israelensis

Vladislav Sazhenskiy; Arieh Zaritsky; Mark Itsko

doi:10.1128/AEM.03068-09

Expression in Escherichia coli of the native cyt1Aa from Bacillus thuringiensis subsp. israelensis

Vladislav Sazhenskiy, Arieh Zaritsky, Mark Itsko

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

The gene cyt1Aa is one of the genes in the complex determining the mosquito larvicidity of Bacillus thuringiensis subsp. israelensis. Previous cloning in Escherichia coli resulted in a 48-bp addition upstream, encoding a chimera. Here, cyt1Aa was recloned without the artifact, and its toxicity against Aedes aegypti larvae and host E. coli cells was retested.

Original language	English
Pages (from-to)	3409-3411
Number of pages	3
Journal	Applied and Environmental Microbiology
Volume	76
Issue number	10
DOIs	https://doi.org/10.1128/AEM.03068-09
State	Published - 1 May 2010

ASJC Scopus subject areas

Biotechnology
Food Science
Applied Microbiology and Biotechnology
Ecology

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abstract = "The gene cyt1Aa is one of the genes in the complex determining the mosquito larvicidity of Bacillus thuringiensis subsp. israelensis. Previous cloning in Escherichia coli resulted in a 48-bp addition upstream, encoding a chimera. Here, cyt1Aa was recloned without the artifact, and its toxicity against Aedes aegypti larvae and host E. coli cells was retested.",

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Expression in Escherichia coli of the native cyt1Aa from Bacillus thuringiensis subsp. israelensis

Abstract

ASJC Scopus subject areas

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