Expression in escherichia coli of the native cyt1Aa from bacillus thuringiensis subsp. israelensis

Vladislav Sazhenskiy; Arieh Zaritsky; Mark Itsko

doi:10.1128/AEM.03068-09

Expression in escherichia coli of the native cyt1Aa from bacillus thuringiensis subsp. israelensis

Vladislav Sazhenskiy
, Arieh Zaritsky
, Mark Itsko

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

The gene cyt1Aa is one of the genes in the complex determining the mosquito larvicidity of Bacillus thuringiensis subsp. israelensis. Previous cloning in Escherichia coli resulted in a 48-bp addition upstream, encoding a chimera. Here, cyt1Aa was recloned without the artifact, and its toxicity against Aedes aegypti larvae and host E. coli cells was retested.

Original language	English
Pages (from-to)	3409-3411
Number of pages	3
Journal	Applied and Environmental Microbiology
Volume	76
Issue number	10
DOIs	https://doi.org/10.1128/AEM.03068-09
State	Published - 1 May 2010

ASJC Scopus subject areas

Biotechnology
Food Science
Applied Microbiology and Biotechnology
Ecology

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title = "Expression in escherichia coli of the native cyt1Aa from bacillus thuringiensis subsp. israelensis",

abstract = "The gene cyt1Aa is one of the genes in the complex determining the mosquito larvicidity of Bacillus thuringiensis subsp. israelensis. Previous cloning in Escherichia coli resulted in a 48-bp addition upstream, encoding a chimera. Here, cyt1Aa was recloned without the artifact, and its toxicity against Aedes aegypti larvae and host E. coli cells was retested.",

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AU - Itsko, Mark

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AB - The gene cyt1Aa is one of the genes in the complex determining the mosquito larvicidity of Bacillus thuringiensis subsp. israelensis. Previous cloning in Escherichia coli resulted in a 48-bp addition upstream, encoding a chimera. Here, cyt1Aa was recloned without the artifact, and its toxicity against Aedes aegypti larvae and host E. coli cells was retested.

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Expression in escherichia coli of the native cyt1Aa from bacillus thuringiensis subsp. israelensis

Abstract

ASJC Scopus subject areas

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