Expression in Escherichia coli of the native cyt1Aa from Bacillus thuringiensis subsp. israelensis

Vladislav Sazhenskiy, Arieh Zaritsky, Mark Itsko

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

The gene cyt1Aa is one of the genes in the complex determining the mosquito larvicidity of Bacillus thuringiensis subsp. israelensis. Previous cloning in Escherichia coli resulted in a 48-bp addition upstream, encoding a chimera. Here, cyt1Aa was recloned without the artifact, and its toxicity against Aedes aegypti larvae and host E. coli cells was retested.

Original languageEnglish
Pages (from-to)3409-3411
Number of pages3
JournalApplied and Environmental Microbiology
Volume76
Issue number10
DOIs
StatePublished - 1 May 2010

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