Expression of coat protein gene of Cucumber mosaic virus (CMV-subgroup IA) Gladiolus isolate in Nicotiana tabacum

Vimal Kumar Dubey; Kottakota Chandrasekhar; Alok Srivastava; Aminuddin; Vijai Pal Singh; Kartik Dhar; Pankaj Kumar Arora

doi:10.1080/17429145.2015.1101496

Expression of coat protein gene of Cucumber mosaic virus (CMV-subgroup IA) Gladiolus isolate in Nicotiana tabacum

Vimal Kumar Dubey, Kottakota Chandrasekhar, Alok Srivastava, Aminuddin, Vijai Pal Singh, Kartik Dhar, Pankaj Kumar Arora

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

The coat protein (CP)-mediated resistance against Cucumber mosaic virus (CMV) subgroup IA was developed in transgenic lines of Nicotiana tabacum cv. Petit Havana using Agrobacterium tumefaciens-mediated transformation. Ten independently transformed lines have developed, four of which were tested for resistance against CMV using virus challenge inoculations. The transgenic lines exhibiting complete resistance remained healthy and symptomless in their life span and showed reduced or no virus accumulation in their systemic leaves after virus challenge inoculation. These transgenic lines also showed resistance against CMV strains which are not closely related to CMV-Gladiolus strains. This is the first report of CP-mediated transgenic resistance against a CMV subgroup IA member isolated from India showing resistance to all CMV strains occurring in the same vicinity.

Original language	English
Pages (from-to)	296-304
Number of pages	9
Journal	Journal of Plant Interactions
Volume	10
Issue number	1
DOIs	https://doi.org/10.1080/17429145.2015.1101496
State	Published - 1 Jan 2015
Externally published	Yes

Keywords

Agromediated transformation
Coat protein mediated resistance
Cucumber mosaic virus
Pathogen derived resistance

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics
Plant Science

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10.1080/17429145.2015.1101496

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title = "Expression of coat protein gene of Cucumber mosaic virus (CMV-subgroup IA) Gladiolus isolate in Nicotiana tabacum",

abstract = "The coat protein (CP)-mediated resistance against Cucumber mosaic virus (CMV) subgroup IA was developed in transgenic lines of Nicotiana tabacum cv. Petit Havana using Agrobacterium tumefaciens-mediated transformation. Ten independently transformed lines have developed, four of which were tested for resistance against CMV using virus challenge inoculations. The transgenic lines exhibiting complete resistance remained healthy and symptomless in their life span and showed reduced or no virus accumulation in their systemic leaves after virus challenge inoculation. These transgenic lines also showed resistance against CMV strains which are not closely related to CMV-Gladiolus strains. This is the first report of CP-mediated transgenic resistance against a CMV subgroup IA member isolated from India showing resistance to all CMV strains occurring in the same vicinity.",

keywords = "Agromediated transformation, Coat protein mediated resistance, Cucumber mosaic virus, Pathogen derived resistance",

author = "Dubey, {Vimal Kumar} and Kottakota Chandrasekhar and Alok Srivastava and Aminuddin and Singh, {Vijai Pal} and Kartik Dhar and Arora, {Pankaj Kumar}",

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doi = "10.1080/17429145.2015.1101496",

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AU - Dubey, Vimal Kumar

AU - Chandrasekhar, Kottakota

AU - Srivastava, Alok

AU - Aminuddin,

AU - Singh, Vijai Pal

AU - Dhar, Kartik

AU - Arora, Pankaj Kumar

PY - 2015/1/1

Y1 - 2015/1/1

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AB - The coat protein (CP)-mediated resistance against Cucumber mosaic virus (CMV) subgroup IA was developed in transgenic lines of Nicotiana tabacum cv. Petit Havana using Agrobacterium tumefaciens-mediated transformation. Ten independently transformed lines have developed, four of which were tested for resistance against CMV using virus challenge inoculations. The transgenic lines exhibiting complete resistance remained healthy and symptomless in their life span and showed reduced or no virus accumulation in their systemic leaves after virus challenge inoculation. These transgenic lines also showed resistance against CMV strains which are not closely related to CMV-Gladiolus strains. This is the first report of CP-mediated transgenic resistance against a CMV subgroup IA member isolated from India showing resistance to all CMV strains occurring in the same vicinity.

KW - Agromediated transformation

KW - Coat protein mediated resistance

KW - Cucumber mosaic virus

KW - Pathogen derived resistance

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Expression of coat protein gene of Cucumber mosaic virus (CMV-subgroup IA) Gladiolus isolate in Nicotiana tabacum

Abstract

Keywords

ASJC Scopus subject areas

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