Expression of glutamine/glutamic acid-rich proteins in rat submandibular glands

L. Mirels; L. Kopec; C. Yagil; D. P. Dickinson; M. Dziejman; L. A. Tabak

doi:10.1016/0003-9969(90)90105-J

Expression of glutamine/glutamic acid-rich proteins in rat submandibular glands

L. Mirels, L. Kopec, C. Yagil, D. P. Dickinson, M. Dziejman, L. A. Tabak

Research output: Contribution to journal › Article › peer-review

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Abstract

The expression of GRP transcripts was found to be highly specific to the rat submandibular gland. GRP cross-reactive species were detected in the saliva of both inbred and outbred rat strains. There was no evidence of GRP transcripts in RNA prepared from bovine, ovine, porcine or murine submandibular glands. Thus the GRPs differ from the family of PRPs that are expressed in several species. The restriction of GRP expression to the rat suggests a relatively recent origin for a functional GRP gene, presumably after rat-mouse divergence. The ontogeny of the relative steady-state levels of GRP transcripts was assessed by dot blot analysis. Maximal levels of RNA-encoding GRP were detected at 6 months; there was a significant age-related decline at both 12 and 18 months. There was, however, no significant age-related alteration in the size of transcripts which encode this protein family.

Original language	English
Pages (from-to)	1-5
Number of pages	5
Journal	Archives of Oral Biology
Volume	35
Issue number	1
DOIs	https://doi.org/10.1016/0003-9969(90)90105-J
State	Published - 1 Jan 1990
Externally published	Yes

Keywords

ageing
evolution of salivary proteins
mucous cells
salivary proteins
tissue-specific gene expression

ASJC Scopus subject areas

Otorhinolaryngology
Dentistry (all)
Cell Biology

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10.1016/0003-9969(90)90105-J

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title = "Expression of glutamine/glutamic acid-rich proteins in rat submandibular glands",

abstract = "The expression of GRP transcripts was found to be highly specific to the rat submandibular gland. GRP cross-reactive species were detected in the saliva of both inbred and outbred rat strains. There was no evidence of GRP transcripts in RNA prepared from bovine, ovine, porcine or murine submandibular glands. Thus the GRPs differ from the family of PRPs that are expressed in several species. The restriction of GRP expression to the rat suggests a relatively recent origin for a functional GRP gene, presumably after rat-mouse divergence. The ontogeny of the relative steady-state levels of GRP transcripts was assessed by dot blot analysis. Maximal levels of RNA-encoding GRP were detected at 6 months; there was a significant age-related decline at both 12 and 18 months. There was, however, no significant age-related alteration in the size of transcripts which encode this protein family.",

keywords = "ageing, evolution of salivary proteins, mucous cells, salivary proteins, tissue-specific gene expression",

author = "L. Mirels and L. Kopec and C. Yagil and Dickinson, {D. P.} and M. Dziejman and Tabak, {L. A.}",

note = "Funding Information: Acknowledgemenrs-This work was supported in part by National Institute of H:ealth Grant DE-08108 (to Lawrence A. Tabak) and P50 DE07003. Lawrence A. Tabak is the recipient of Research Career Development Award DE-00217. Lily Mirels was supported at SUNYAB by Training Grant DE-07034. We thank Dr G. S. Bedi for his preparation of the monoclonal antibody. We are most grateful to Dr Bruce Baum (PCCIB, NIDR/NIH) who provided us with tissues from aged animals, critically reviewed this manuscript and encouraged us. The authors also wish to thank Susan Lukas and Beth Tollers for typing the manuscript.",

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doi = "10.1016/0003-9969(90)90105-J",

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AU - Dziejman, M.

AU - Tabak, L. A.

N1 - Funding Information: Acknowledgemenrs-This work was supported in part by National Institute of H:ealth Grant DE-08108 (to Lawrence A. Tabak) and P50 DE07003. Lawrence A. Tabak is the recipient of Research Career Development Award DE-00217. Lily Mirels was supported at SUNYAB by Training Grant DE-07034. We thank Dr G. S. Bedi for his preparation of the monoclonal antibody. We are most grateful to Dr Bruce Baum (PCCIB, NIDR/NIH) who provided us with tissues from aged animals, critically reviewed this manuscript and encouraged us. The authors also wish to thank Susan Lukas and Beth Tollers for typing the manuscript.

PY - 1990/1/1

Y1 - 1990/1/1

N2 - The expression of GRP transcripts was found to be highly specific to the rat submandibular gland. GRP cross-reactive species were detected in the saliva of both inbred and outbred rat strains. There was no evidence of GRP transcripts in RNA prepared from bovine, ovine, porcine or murine submandibular glands. Thus the GRPs differ from the family of PRPs that are expressed in several species. The restriction of GRP expression to the rat suggests a relatively recent origin for a functional GRP gene, presumably after rat-mouse divergence. The ontogeny of the relative steady-state levels of GRP transcripts was assessed by dot blot analysis. Maximal levels of RNA-encoding GRP were detected at 6 months; there was a significant age-related decline at both 12 and 18 months. There was, however, no significant age-related alteration in the size of transcripts which encode this protein family.

AB - The expression of GRP transcripts was found to be highly specific to the rat submandibular gland. GRP cross-reactive species were detected in the saliva of both inbred and outbred rat strains. There was no evidence of GRP transcripts in RNA prepared from bovine, ovine, porcine or murine submandibular glands. Thus the GRPs differ from the family of PRPs that are expressed in several species. The restriction of GRP expression to the rat suggests a relatively recent origin for a functional GRP gene, presumably after rat-mouse divergence. The ontogeny of the relative steady-state levels of GRP transcripts was assessed by dot blot analysis. Maximal levels of RNA-encoding GRP were detected at 6 months; there was a significant age-related decline at both 12 and 18 months. There was, however, no significant age-related alteration in the size of transcripts which encode this protein family.

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KW - mucous cells

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KW - tissue-specific gene expression

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Expression of glutamine/glutamic acid-rich proteins in rat submandibular glands

Abstract

Keywords

ASJC Scopus subject areas

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