Expression of GTP-dependent and GTP-independent tissue-type transglutaminase in cytokine-treated rat brain astrocytes

Alon Monsonego, Yael Shani, Igor Friedmann, Yoav Paas, Orly Eizenberg, Michal Schwartz

Research output: Contribution to journalArticlepeer-review

93 Scopus citations

Abstract

Tissue-type transglutaminases (TGases) were recently shown to exert dual enzymatic activities; they catalyze the posttranslational modification of proteins by transamidation, and they also act as guanosine triphosphatase (GTPase). Here we show that a tissue-type TGase is expressed in rat brain astrocytes in vitro, and is induced by the inflammation-associated cytokines interleukin-1β and to a lesser extent by tumor necrosis factor-α. Induction is accompanied by overexpression and appearance of an additional shorter clone, which does not contain the long 3'-untranslated region and encodes for a novel TGase enzyme whose C terminus lacks a site that affects the enzyme's interaction with guanosine triphosphate (GTP). Expression of two clones revealed that the long form is inhibited noncompetitively by GTP, but the short form significantly less so. The different affinities for GTP may account for the difference in physiological function between these two enzymes.

Original languageEnglish
Pages (from-to)3724-3732
Number of pages9
JournalJournal of Biological Chemistry
Volume272
Issue number6
DOIs
StatePublished - 20 Feb 1997
Externally publishedYes

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