TY - JOUR
T1 - Expression of TLR2 and TLR4 on peripheral blood monocytes during exacerbation of atopic dermatitis
AU - Tsybikov, Namjil N.
AU - Petrisheva, Irina
AU - Fefelova, Elena V.
AU - Kuznik, Boris I.
AU - Magen, Eli
N1 - Publisher Copyright:
© 2015, OceanSide Publications, Inc., U.S.A.
PY - 2015/11/1
Y1 - 2015/11/1
N2 - Background: In atopic dermatitis (AD), monocytes, which accumulate in the inflamed skin, are characterized by a significantly impaired Toll-like receptors (TLR) expression and TLR2-mediated cytokine secretion. However, data on expression of TLR on monocytes of peripheral blood (PB) in AD are not available. Objective: To investigate TLR2 and TLR4 expression on PB monocytes during AD exacerbation and to assess the relationships between TLR expressions with AD clinical severity and with serum interleukin (IL) 4, IL-10, and IL-17a levels. Methods: The objective Scoring Atopic Dermatitis index, TLR2 and TLR4 expression on CD14+ human leukocyte antigen-DR (HLA-DR-) PB monocytes by flow cytometry, serum IL-4, IL-10, IL-17a (enzyme-linked immunosorbent assay) and total immunoglobulin E levels were measured at study entry and after 4 months in patients with AD and healthy controls. Results: Eighty-Two patients with AD, 35 women (45.1%) and 47 men (54.9%), mean (standard deviation [SD]) age, 42.2 ± 11.5 years, were included. Thirty healthy volunteers served as controls. We observed a significant difference in the levels of TLR2 expression in the CD14+ HLA-DR+ PB monocytes of patients with AD (mean [SD], 51.6 ± 23.1% and 264 ± 118 cells/mm3) at exacerbation (but not at the end of the 4-month postexacerbation period) compared with the healthy control subjects (mean [SD], 22.3 ± 10.6% and 105 ± 50 cells/mm3; p < 0.001). TLR4 expression in PB monocytes was significantly greater in AD (mean [SD], 50.1 ± 20.9% and 275 ± 114 cells/mm3) than in the healthy subjects (mean [SD], 31.2 ± 8.7% and 147 ± 41 cells/mm3; p < 0.001) both at exacerbation and at the 4-month postexacerbation period. Significant correlations between TLR2+ (but not TLR4+) PB monocytes and the objective Scoring Atopic Dermatitis index (r = 0.604, p < 0.001), serum levels of IL-17a and TLR2- PB monocytes (r = 0.416, p ± 0.027), and IL-4 and TLR2- PB monocytes (r = =0.307, p = 0.014) were observed during AD exacerbation. Conclusion: PB CD14+ HLA-DR+ TLR2+ monocytes might have a role in the skewing of a T-helper 2/T-helper 17-mediated immune response during AD flare.
AB - Background: In atopic dermatitis (AD), monocytes, which accumulate in the inflamed skin, are characterized by a significantly impaired Toll-like receptors (TLR) expression and TLR2-mediated cytokine secretion. However, data on expression of TLR on monocytes of peripheral blood (PB) in AD are not available. Objective: To investigate TLR2 and TLR4 expression on PB monocytes during AD exacerbation and to assess the relationships between TLR expressions with AD clinical severity and with serum interleukin (IL) 4, IL-10, and IL-17a levels. Methods: The objective Scoring Atopic Dermatitis index, TLR2 and TLR4 expression on CD14+ human leukocyte antigen-DR (HLA-DR-) PB monocytes by flow cytometry, serum IL-4, IL-10, IL-17a (enzyme-linked immunosorbent assay) and total immunoglobulin E levels were measured at study entry and after 4 months in patients with AD and healthy controls. Results: Eighty-Two patients with AD, 35 women (45.1%) and 47 men (54.9%), mean (standard deviation [SD]) age, 42.2 ± 11.5 years, were included. Thirty healthy volunteers served as controls. We observed a significant difference in the levels of TLR2 expression in the CD14+ HLA-DR+ PB monocytes of patients with AD (mean [SD], 51.6 ± 23.1% and 264 ± 118 cells/mm3) at exacerbation (but not at the end of the 4-month postexacerbation period) compared with the healthy control subjects (mean [SD], 22.3 ± 10.6% and 105 ± 50 cells/mm3; p < 0.001). TLR4 expression in PB monocytes was significantly greater in AD (mean [SD], 50.1 ± 20.9% and 275 ± 114 cells/mm3) than in the healthy subjects (mean [SD], 31.2 ± 8.7% and 147 ± 41 cells/mm3; p < 0.001) both at exacerbation and at the 4-month postexacerbation period. Significant correlations between TLR2+ (but not TLR4+) PB monocytes and the objective Scoring Atopic Dermatitis index (r = 0.604, p < 0.001), serum levels of IL-17a and TLR2- PB monocytes (r = 0.416, p ± 0.027), and IL-4 and TLR2- PB monocytes (r = =0.307, p = 0.014) were observed during AD exacerbation. Conclusion: PB CD14+ HLA-DR+ TLR2+ monocytes might have a role in the skewing of a T-helper 2/T-helper 17-mediated immune response during AD flare.
UR - http://www.scopus.com/inward/record.url?scp=84948703383&partnerID=8YFLogxK
U2 - 10.2500/aap.2015.36.3901
DO - 10.2500/aap.2015.36.3901
M3 - Article
AN - SCOPUS:84948703383
SN - 1088-5412
VL - 36
SP - e140-e145
JO - Allergy and Asthma Proceedings
JF - Allergy and Asthma Proceedings
IS - 6
ER -