Expression of two inward rectifier potassium channels is essential for differentiation of primitive human hematopoietic progenitor cells

Orian Shirihai, Bernard Attali, Daniel Dagan, Shoshana Merchav

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

A potassium inward rectifier (K(it)) current was previously shown by us to be induced in primitive hematopoietic progenitor cells, stimulated with the combination of interleukin-3 (IL-3) and stem cell factor (SCF). Biophysical features of whole cell currents implicated the involvement of more than one K(it) channel type. Employing IL-3 + SCF stimulated human cord blood CD34+38- cells, we isolated and characterized different components of this current. Reverse transcription-polymerase chain reaction (RT-PCR) subcloning identified the expression of a strongly rectifying K(ir) channel (K(ir) 4.3) as well as a weakly rectifying K(ir) channel (K(ir) 1.1) in these cells. Inhibition of the expression of each of the channels suppressed progenitor cell generation by IL-3 and SCF-stimulated CD34+38- cells in 7- day suspension cultures. The variable expression of two essential inward rectifying potassium channels early in the course of hematopoietic progenitor cell differentiation may play a potentially important role in potassium homeostasis in these cells.

Original languageEnglish
Pages (from-to)197-205
Number of pages9
JournalJournal of Cellular Physiology
Volume177
Issue number2
DOIs
StatePublished - 1 Nov 1998
Externally publishedYes

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