Expression profiling of constitutive mast cells reveals a unique identity within the immune system

Daniel F. Dwyer; Nora A. Barrett; K. Frank Austen; Edy Y. Kim; Michael B. Brenner; Laura Shaw; Bingfei Yu; Ananda Goldrath; Sara Mostafavi; Aviv Regev; Andrew Rhoades; Devapregasan Moodley; Hideyuki Yoshida; Diane Mathis; Christophe Benoist; Tsukasa Nabekura; Viola Lam; Lewis L. Lanier; Brian Brown; Miriam Merad; Viviana Cremasco; Shannon Turley; Paul Monach; Michael L. Dustin; Yuesheng Li; Susan A. Shinton; Richard R. Hardy; Tal Shay; Yilin Qi; Katelyn Sylvia; Joonsoo Kang; Keke Fairfax; Gwendalyn J. Randolph; Michelle L. Robinette; Anja Fuchs; Marco Colonna

doi:10.1038/ni.3445

Expression profiling of constitutive mast cells reveals a unique identity within the immune system

Daniel F. Dwyer, Nora A. Barrett, K. Frank Austen, Edy Y. Kim, Michael B. Brenner, Laura Shaw, Bingfei Yu, Ananda Goldrath, Sara Mostafavi, Aviv Regev, Andrew Rhoades, Devapregasan Moodley, Hideyuki Yoshida, Diane Mathis, Christophe Benoist, Tsukasa Nabekura, Viola Lam, Lewis L. Lanier, Brian Brown, Miriam MeradViviana Cremasco, Shannon Turley, Paul Monach, Michael L. Dustin, Yuesheng Li, Susan A. Shinton, Richard R. Hardy, Tal Shay, Yilin Qi, Katelyn Sylvia, Joonsoo Kang, Keke Fairfax, Gwendalyn J. Randolph, Michelle L. Robinette, Anja Fuchs, Marco Colonna

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

276 Scopus citations

Abstract

Mast cells are evolutionarily ancient sentinel cells. Like basophils, mast cells express the high-affinity receptor for immunoglobulin E (IgE) and have been linked to host defense and diverse immune-system-mediated diseases. To better characterize the function of these cells, we assessed the transcriptional profiles of mast cells isolated from peripheral connective tissues and basophils isolated from spleen and blood. We found that mast cells were transcriptionally distinct, clustering independently from all other profiled cells, and that mast cells demonstrated considerably greater heterogeneity across tissues than previously appreciated. We observed minimal homology between mast cells and basophils, which shared more overlap with other circulating granulocytes than with mast cells. The derivation of mast-cell and basophil transcriptional signatures underscores their differential capacities to detect environmental signals and influence the inflammatory milieu.

Original language	English
Pages (from-to)	878-887
Number of pages	10
Journal	Nature Immunology
Volume	17
Issue number	7
DOIs	https://doi.org/10.1038/ni.3445
State	Published - 21 Jun 2016

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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Dwyer, D. F., Barrett, N. A., Austen, K. F., Kim, E. Y., Brenner, M. B., Shaw, L., Yu, B., Goldrath, A., Mostafavi, S., Regev, A., Rhoades, A., Moodley, D., Yoshida, H., Mathis, D., Benoist, C., Nabekura, T., Lam, V., Lanier, L. L., Brown, B., ... Colonna, M. (2016). Expression profiling of constitutive mast cells reveals a unique identity within the immune system. Nature Immunology, 17(7), 878-887. https://doi.org/10.1038/ni.3445

@article{6f675f42021042e481d096fc97ed2195,

title = "Expression profiling of constitutive mast cells reveals a unique identity within the immune system",

abstract = "Mast cells are evolutionarily ancient sentinel cells. Like basophils, mast cells express the high-affinity receptor for immunoglobulin E (IgE) and have been linked to host defense and diverse immune-system-mediated diseases. To better characterize the function of these cells, we assessed the transcriptional profiles of mast cells isolated from peripheral connective tissues and basophils isolated from spleen and blood. We found that mast cells were transcriptionally distinct, clustering independently from all other profiled cells, and that mast cells demonstrated considerably greater heterogeneity across tissues than previously appreciated. We observed minimal homology between mast cells and basophils, which shared more overlap with other circulating granulocytes than with mast cells. The derivation of mast-cell and basophil transcriptional signatures underscores their differential capacities to detect environmental signals and influence the inflammatory milieu.",

author = "Dwyer, {Daniel F.} and Barrett, {Nora A.} and Austen, {K. Frank} and Kim, {Edy Y.} and Brenner, {Michael B.} and Laura Shaw and Bingfei Yu and Ananda Goldrath and Sara Mostafavi and Aviv Regev and Andrew Rhoades and Devapregasan Moodley and Hideyuki Yoshida and Diane Mathis and Christophe Benoist and Tsukasa Nabekura and Viola Lam and Lanier, {Lewis L.} and Brian Brown and Miriam Merad and Viviana Cremasco and Shannon Turley and Paul Monach and Dustin, {Michael L.} and Yuesheng Li and Shinton, {Susan A.} and Hardy, {Richard R.} and Tal Shay and Yilin Qi and Katelyn Sylvia and Joonsoo Kang and Keke Fairfax and Randolph, {Gwendalyn J.} and Robinette, {Michelle L.} and Anja Fuchs and Marco Colonna",

year = "2016",

month = jun,

day = "21",

doi = "10.1038/ni.3445",

language = "English",

volume = "17",

pages = "878--887",

journal = "Nature Immunology",

issn = "1529-2908",

publisher = "Nature Publishing Group",

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Dwyer, DF, Barrett, NA, Austen, KF, Kim, EY, Brenner, MB, Shaw, L, Yu, B, Goldrath, A, Mostafavi, S, Regev, A, Rhoades, A, Moodley, D, Yoshida, H, Mathis, D, Benoist, C, Nabekura, T, Lam, V, Lanier, LL, Brown, B, Merad, M, Cremasco, V, Turley, S, Monach, P, Dustin, ML, Li, Y, Shinton, SA, Hardy, RR, Shay, T, Qi, Y, Sylvia, K, Kang, J, Fairfax, K, Randolph, GJ, Robinette, ML, Fuchs, A & Colonna, M 2016, 'Expression profiling of constitutive mast cells reveals a unique identity within the immune system', Nature Immunology, vol. 17, no. 7, pp. 878-887. https://doi.org/10.1038/ni.3445

TY - JOUR

T1 - Expression profiling of constitutive mast cells reveals a unique identity within the immune system

AU - Dwyer, Daniel F.

AU - Barrett, Nora A.

AU - Austen, K. Frank

AU - Kim, Edy Y.

AU - Brenner, Michael B.

AU - Shaw, Laura

AU - Yu, Bingfei

AU - Goldrath, Ananda

AU - Mostafavi, Sara

AU - Regev, Aviv

AU - Rhoades, Andrew

AU - Moodley, Devapregasan

AU - Yoshida, Hideyuki

AU - Mathis, Diane

AU - Benoist, Christophe

AU - Nabekura, Tsukasa

AU - Lam, Viola

AU - Lanier, Lewis L.

AU - Brown, Brian

AU - Merad, Miriam

AU - Cremasco, Viviana

AU - Turley, Shannon

AU - Monach, Paul

AU - Dustin, Michael L.

AU - Li, Yuesheng

AU - Shinton, Susan A.

AU - Hardy, Richard R.

AU - Shay, Tal

AU - Qi, Yilin

AU - Sylvia, Katelyn

AU - Kang, Joonsoo

AU - Fairfax, Keke

AU - Randolph, Gwendalyn J.

AU - Robinette, Michelle L.

AU - Fuchs, Anja

AU - Colonna, Marco

PY - 2016/6/21

Y1 - 2016/6/21

N2 - Mast cells are evolutionarily ancient sentinel cells. Like basophils, mast cells express the high-affinity receptor for immunoglobulin E (IgE) and have been linked to host defense and diverse immune-system-mediated diseases. To better characterize the function of these cells, we assessed the transcriptional profiles of mast cells isolated from peripheral connective tissues and basophils isolated from spleen and blood. We found that mast cells were transcriptionally distinct, clustering independently from all other profiled cells, and that mast cells demonstrated considerably greater heterogeneity across tissues than previously appreciated. We observed minimal homology between mast cells and basophils, which shared more overlap with other circulating granulocytes than with mast cells. The derivation of mast-cell and basophil transcriptional signatures underscores their differential capacities to detect environmental signals and influence the inflammatory milieu.

AB - Mast cells are evolutionarily ancient sentinel cells. Like basophils, mast cells express the high-affinity receptor for immunoglobulin E (IgE) and have been linked to host defense and diverse immune-system-mediated diseases. To better characterize the function of these cells, we assessed the transcriptional profiles of mast cells isolated from peripheral connective tissues and basophils isolated from spleen and blood. We found that mast cells were transcriptionally distinct, clustering independently from all other profiled cells, and that mast cells demonstrated considerably greater heterogeneity across tissues than previously appreciated. We observed minimal homology between mast cells and basophils, which shared more overlap with other circulating granulocytes than with mast cells. The derivation of mast-cell and basophil transcriptional signatures underscores their differential capacities to detect environmental signals and influence the inflammatory milieu.

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DO - 10.1038/ni.3445

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C2 - 27135604

AN - SCOPUS:84965019138

SN - 1529-2908

VL - 17

SP - 878

EP - 887

JO - Nature Immunology

JF - Nature Immunology

IS - 7

ER -

Expression profiling of constitutive mast cells reveals a unique identity within the immune system

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