Abstract
Axial resolution is one of the most important characteristics of a microscope. In all microscopes, a high axial resolution is desired in order to discriminate information efficiently along the longitudinal direction. However, when studying thick samples that do not contain laterally overlapping information, a low axial resolution is desirable, as information from multiple planes can be recorded simultaneously from a single camera shot instead of plane-by-plane mechanical refocusing. In this study, we increased the focal depth of an infrared microscope non-invasively by introducing a binary axicon fabricated on a barium fluoride substrate close to the sample. Preliminary results of imaging the thick and sparse silk fibers showed an improved focal depth with a slight decrease in lateral resolution and an increase in background noise.
Original language | English |
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Article number | 537 |
Journal | Micromachines |
Volume | 15 |
Issue number | 4 |
DOIs | |
State | Published - 1 Apr 2024 |
Externally published | Yes |
Keywords
- axial resolution
- binary axicon
- chemical imaging
- femtosecond ablation
- infrared microscopy
- photolithography
ASJC Scopus subject areas
- Control and Systems Engineering
- Mechanical Engineering
- Electrical and Electronic Engineering