@article{ca04dbf0dea64ac08a8de3267e74fcad,
title = "Fabrication of organic phase biosensors based on multilayered polyphenol oxidase protected by an alginate coating",
abstract = "We describe herein, the creation of an organic phase enzyme electrode (OPEE) via avidin-biotin interactions built over an electrogenerated polymer. Multilayered polyphenol oxidase (PPO) assemblies were transferred into an organic solvent (chloroform) for the catechol detection at -0.2 V. In conjunction with an alginate gel, as a hydrophilic additive, the biosensor performance was widely enhanced. The effects of biotinylated polypyrrole film and alginate gel on the diffusion process through the biosensor coating are studied by rotating disk electrode experiments carried out in chloroform with hydroquinone as electroactive permeant.",
keywords = "Alginate, Avidin-biotin, Biosensor, Organic phase, Polyphenol oxidase, Polypyrrole",
author = "Christine Mousty and Arielle Lepellec and Serge Cosnier and Andres Novoa and Marks, {Robert S.}",
note = "Funding Information: Drs. Serge Cosnier, Robert S. Marks and Andres Novoa would like to thank the French Minist{\`e}re des Affaires Etrang{\`e}res and the Israel Ministry of Science, the Arts and Sports, for their travel grant support under the Binational France–Israel Arc-en-Ciel/Keshet programme. They also wish to thank the Commission of the European Communities Research Directorate for their support under the PEBCAT project contract No. EVK1-CT-2000-00069. Andr{\'e}s Novoa thanks the Israel Ministry of Absorption for partial support. Dr. Robert S. Marks thanks the Israel Ministry of Science, Culture and Sport for the infrastructure grant # 85902201.",
year = "2001",
month = dec,
day = "10",
doi = "10.1016/S1388-2481(01)00252-1",
language = "English",
volume = "3",
pages = "727--732",
journal = "Electrochemistry Communications",
issn = "1388-2481",
publisher = "Elsevier Inc.",
number = "12",
}