Fabrification of electroreduced graphene oxide-bentonite sodium composite modified electrode and its sensing application for linezolid

S. N. Prashanth; Nagappa L. Teradal; J. Seetharamappa; Ashis K. Satpati; A. V.R. Reddy

doi:10.1016/j.electacta.2014.04.022

Fabrification of electroreduced graphene oxide-bentonite sodium composite modified electrode and its sensing application for linezolid

S. N. Prashanth, Nagappa L. Teradal, J. Seetharamappa, Ashis K. Satpati, A. V.R. Reddy

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

Graphene and its composites have attracted considerable attention in synthesis and electrochemical applications. In the present work, we have synthesized and characterized graphene oxide-bentonite composite (GO-BEN) and utilized it to fabricate an electrochemical sensor. For this, the solution of GO-BEN cast on glassy carbon electrode (GCE) was reduced electrochemically in phosphate buffer solution of pH 6 to obtain electrochemically reduced graphene oxide-bentonite composite (ERGO-BEN-GCE). This ERGO-BEN film was used for electrochemical investigation of an oxazolidinone class of antibiotic, linezolid (LIN) for the first time. The electrochemical sensor showed excellent enhancement and adsorptive ability towards the electrooxidation of LIN. LIN exhibited two each of oxidation and reduction peaks on ERGO-BEN film in phosphate buffer of pH 7.0. Effects of accumulation time, pH of solution and scan rate were studied and various electrochemical parameters were evaluated. The plot of pH versus E_p gave a slope of 26.2 mV/pH in the pH range of 4.2-8.0 indicating the participation of two electrons and one proton in the electrode process. An adsorptive stripping differential pulse voltammetric method (AdSDPV) was developed for the determination of LIN in bulk, pharmaceutical formulations and urine samples. Adsorptive stripping linear sweep voltammetric (AdSLSV) and differential pulse voltammetric (DPV) methods were also developed and the results were compared. LIN showed linear relationship between the current density and concentration in the range of 0.25 - 31.25 μM with a LOD of 0.0337 μM in AdSDPV method; 0.5 - 31.25 μM with a LOD of 0.100 μM in DPV method and 1.25 - 37.5 μM with a LOD of 0.5461 μM in AdSLSV method respectively. The proposed AdSDPV method was observed to be simple, fast and inexpensive and hence, could be readily adopted for quality control in pharmaceutical products.

Original language	English
Pages (from-to)	49-56
Number of pages	8
Journal	Electrochimica Acta
Volume	133
DOIs	https://doi.org/10.1016/j.electacta.2014.04.022
State	Published - 1 Jul 2014
Externally published	Yes

Keywords

Bentonite
Electrochemical Sensor
Electroreduction
Graphene
Linezolid

ASJC Scopus subject areas

General Chemical Engineering
Electrochemistry

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10.1016/j.electacta.2014.04.022

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@article{e69d02a2eac44773b58c94d816123ab9,

title = "Fabrification of electroreduced graphene oxide-bentonite sodium composite modified electrode and its sensing application for linezolid",

abstract = "Graphene and its composites have attracted considerable attention in synthesis and electrochemical applications. In the present work, we have synthesized and characterized graphene oxide-bentonite composite (GO-BEN) and utilized it to fabricate an electrochemical sensor. For this, the solution of GO-BEN cast on glassy carbon electrode (GCE) was reduced electrochemically in phosphate buffer solution of pH 6 to obtain electrochemically reduced graphene oxide-bentonite composite (ERGO-BEN-GCE). This ERGO-BEN film was used for electrochemical investigation of an oxazolidinone class of antibiotic, linezolid (LIN) for the first time. The electrochemical sensor showed excellent enhancement and adsorptive ability towards the electrooxidation of LIN. LIN exhibited two each of oxidation and reduction peaks on ERGO-BEN film in phosphate buffer of pH 7.0. Effects of accumulation time, pH of solution and scan rate were studied and various electrochemical parameters were evaluated. The plot of pH versus Ep gave a slope of 26.2 mV/pH in the pH range of 4.2-8.0 indicating the participation of two electrons and one proton in the electrode process. An adsorptive stripping differential pulse voltammetric method (AdSDPV) was developed for the determination of LIN in bulk, pharmaceutical formulations and urine samples. Adsorptive stripping linear sweep voltammetric (AdSLSV) and differential pulse voltammetric (DPV) methods were also developed and the results were compared. LIN showed linear relationship between the current density and concentration in the range of 0.25 - 31.25 μM with a LOD of 0.0337 μM in AdSDPV method; 0.5 - 31.25 μM with a LOD of 0.100 μM in DPV method and 1.25 - 37.5 μM with a LOD of 0.5461 μM in AdSLSV method respectively. The proposed AdSDPV method was observed to be simple, fast and inexpensive and hence, could be readily adopted for quality control in pharmaceutical products.",

keywords = "Bentonite, Electrochemical Sensor, Electroreduction, Graphene, Linezolid",

author = "Prashanth, {S. N.} and Teradal, {Nagappa L.} and J. Seetharamappa and Satpati, {Ashis K.} and Reddy, {A. V.R.}",

note = "Funding Information: We thank the Board of Research in Nuclear Sciences, BARC, Mumbai, for financial support (No.2012/37 C/8/BRNS/637 dated 28-05-2012) to carry out this work. Thanks are also due to the authorities of the Karnatak University, Dharwad, for providing the necessary facilities. ",

year = "2014",

month = jul,

day = "1",

doi = "10.1016/j.electacta.2014.04.022",

language = "English",

volume = "133",

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journal = "Electrochimica Acta",

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T1 - Fabrification of electroreduced graphene oxide-bentonite sodium composite modified electrode and its sensing application for linezolid

AU - Prashanth, S. N.

AU - Teradal, Nagappa L.

AU - Seetharamappa, J.

AU - Satpati, Ashis K.

AU - Reddy, A. V.R.

N1 - Funding Information: We thank the Board of Research in Nuclear Sciences, BARC, Mumbai, for financial support (No.2012/37 C/8/BRNS/637 dated 28-05-2012) to carry out this work. Thanks are also due to the authorities of the Karnatak University, Dharwad, for providing the necessary facilities.

PY - 2014/7/1

Y1 - 2014/7/1

N2 - Graphene and its composites have attracted considerable attention in synthesis and electrochemical applications. In the present work, we have synthesized and characterized graphene oxide-bentonite composite (GO-BEN) and utilized it to fabricate an electrochemical sensor. For this, the solution of GO-BEN cast on glassy carbon electrode (GCE) was reduced electrochemically in phosphate buffer solution of pH 6 to obtain electrochemically reduced graphene oxide-bentonite composite (ERGO-BEN-GCE). This ERGO-BEN film was used for electrochemical investigation of an oxazolidinone class of antibiotic, linezolid (LIN) for the first time. The electrochemical sensor showed excellent enhancement and adsorptive ability towards the electrooxidation of LIN. LIN exhibited two each of oxidation and reduction peaks on ERGO-BEN film in phosphate buffer of pH 7.0. Effects of accumulation time, pH of solution and scan rate were studied and various electrochemical parameters were evaluated. The plot of pH versus Ep gave a slope of 26.2 mV/pH in the pH range of 4.2-8.0 indicating the participation of two electrons and one proton in the electrode process. An adsorptive stripping differential pulse voltammetric method (AdSDPV) was developed for the determination of LIN in bulk, pharmaceutical formulations and urine samples. Adsorptive stripping linear sweep voltammetric (AdSLSV) and differential pulse voltammetric (DPV) methods were also developed and the results were compared. LIN showed linear relationship between the current density and concentration in the range of 0.25 - 31.25 μM with a LOD of 0.0337 μM in AdSDPV method; 0.5 - 31.25 μM with a LOD of 0.100 μM in DPV method and 1.25 - 37.5 μM with a LOD of 0.5461 μM in AdSLSV method respectively. The proposed AdSDPV method was observed to be simple, fast and inexpensive and hence, could be readily adopted for quality control in pharmaceutical products.

AB - Graphene and its composites have attracted considerable attention in synthesis and electrochemical applications. In the present work, we have synthesized and characterized graphene oxide-bentonite composite (GO-BEN) and utilized it to fabricate an electrochemical sensor. For this, the solution of GO-BEN cast on glassy carbon electrode (GCE) was reduced electrochemically in phosphate buffer solution of pH 6 to obtain electrochemically reduced graphene oxide-bentonite composite (ERGO-BEN-GCE). This ERGO-BEN film was used for electrochemical investigation of an oxazolidinone class of antibiotic, linezolid (LIN) for the first time. The electrochemical sensor showed excellent enhancement and adsorptive ability towards the electrooxidation of LIN. LIN exhibited two each of oxidation and reduction peaks on ERGO-BEN film in phosphate buffer of pH 7.0. Effects of accumulation time, pH of solution and scan rate were studied and various electrochemical parameters were evaluated. The plot of pH versus Ep gave a slope of 26.2 mV/pH in the pH range of 4.2-8.0 indicating the participation of two electrons and one proton in the electrode process. An adsorptive stripping differential pulse voltammetric method (AdSDPV) was developed for the determination of LIN in bulk, pharmaceutical formulations and urine samples. Adsorptive stripping linear sweep voltammetric (AdSLSV) and differential pulse voltammetric (DPV) methods were also developed and the results were compared. LIN showed linear relationship between the current density and concentration in the range of 0.25 - 31.25 μM with a LOD of 0.0337 μM in AdSDPV method; 0.5 - 31.25 μM with a LOD of 0.100 μM in DPV method and 1.25 - 37.5 μM with a LOD of 0.5461 μM in AdSLSV method respectively. The proposed AdSDPV method was observed to be simple, fast and inexpensive and hence, could be readily adopted for quality control in pharmaceutical products.

KW - Bentonite

KW - Electrochemical Sensor

KW - Electroreduction

KW - Graphene

KW - Linezolid

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JO - Electrochimica Acta

JF - Electrochimica Acta

ER -

Fabrification of electroreduced graphene oxide-bentonite sodium composite modified electrode and its sensing application for linezolid

Abstract

Keywords

ASJC Scopus subject areas

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