Fatty acid binding protein (FABP) modulates prostaglandin E binding to rat epididymal adipocyte membrane similarly to albumin

Rivka Cohen-Luria, Abraham Danon, Gilad Rimon

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Albumin enhances prostaglandin E2 (PGE2) binding to isolated epididymal adipocyte membrane and also binds PGE2 with low affinity. On the other hand, S-100, ovalbumin and albumin-stearate failed to bind PGE2, as shown by ultrafiltration, and also failed to enhance PGE2 binding to the isolated adipocyte membranes. These results suggested that albumin enhances PGE2 binding possibly by serving as a carrier for the prostaglandin molecules. 3 mM warfarin or 1 mM phenylbutazone inhibited PGE2 binding to albumin by 70% and 95%, respectively, but both drugs failed to affect the enhancement of PGE2 binding to the isolated adipocyte membrane in the presence of albumin. These results exclude the possibility that PGE2 bound to albumin is more accessible to the prostaglandin receptor than free PGE2 in solution. Finally it is shown that fatty acid binding protein (FABP), a cytosolic protein which binds specifically PGE1 but not PGE2, enhances PGE1 and PGE2 binding to isolated adipocyte membranes similarly to albumin. The physiological implications of these findings are discussed.

Original languageEnglish
Pages (from-to)96-100
Number of pages5
JournalBiochimica et Biophysica Acta - Biomembranes
Volume1021
Issue number1
DOIs
StatePublished - 15 Jan 1990

Keywords

  • (Rat)
  • Adipocyte membrane
  • Fatty acid binding protein
  • Prostaglandin E

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