Fetal membranes as an interface between inflammation and metabolism: Increased Aquaporin 9 expression in the presence of spontaneous labor at term and chorioamnionitis

Pooja Mittal, Roberto Romero, Shali Mazaki-Tovi, Gerard Tromp, Adi L. Tarca, Yeon Mee Kim, Tinnakorn Chaiworapongsa, Juan Pedro Kusanovic, Offer Erez, Nandor Gabor Than, Sonia S. Hassan

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Objective. Aquaporin 9 (AQP9) is a water channel protein characterized by its high permeability to nutrients such as lactate and glycerol, as well as urea and other small solutes. These unique properties of AQP9 suggest that this molecule may play a role in the modulation of nutrient flux through the fetal membranes in conditions associated with increased metabolic demand, such as spontaneous labor and inflammation. The objective of this study was to determine the expression of AQP9 in the chorioamniotic membranes from women with and without term labor, as well as those with preterm prelabor rupture of membranes (PPROM) with and without histologic chorioamnionitis. Study design. A cross-sectional study was performed which included patients in the following groups: (1) term not in labor (TNL; n=14); (2) term, spontaneous labor (n=14); and (3) PPROM with (n20) and without (n=17) histologic chorioamnionitis. AQP9 mRNA expression in fetal membranes was quantified using quantitative real-time reverse transcription-polymerase chain reaction and analyzed with a linear model including gestational age as a covariate. Results. (1) AQP9 mRNA expression was identified in all chorioamniotic membrane specimens; (2) AQP9 expression in fetal membranes was significantly higher in spontaneous term labor when compared with TNL (fold change 3.6; p=0.01); and (3) Among patients with PPROM, the presence of histologic chorioamnionitis was associated with a higher expression of AQP9 in the chorioamniotic membranes compared with those from women without histologic chorioamnionitis (fold change 8.7; p<0.001). Conclusion. Aquaporin 9 mRNA expression is higher in the fetal membranes from patients with spontaneous term labor and those with PPROM and histologic chorioamnionitis. These findings are novel, and suggest a role for aquaporin 9 in membrane-mediated transfer of nutrients to support the increased metabolic demands associated with the host immune response of the terminal pathway of parturition and histologic chorioamnionitis.

Original languageEnglish
Pages (from-to)1167-1175
Number of pages9
JournalJournal of Maternal-Fetal and Neonatal Medicine
Volume22
Issue number12
DOIs
StatePublished - 1 Dec 2009
Externally publishedYes

Keywords

  • Aquaporin 9
  • Chorioamniotic membranes
  • Intra-amniotic infection/inflammation
  • Metabolism
  • Microarray
  • PCR
  • Parturition
  • Pregnancy
  • Premature birth
  • Preterm prelabor rupture of membranes
  • Water channels

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Obstetrics and Gynecology

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